Multivesicular release at climbing fiber-Purkinje cell synapses

Jacques I. Wadiche, Craig Jahr

Research output: Contribution to journalArticle

276 Citations (Scopus)

Abstract

Synapses driven by action potentials are thought to release transmitter in an all-or-none fashion; either one synaptic vesicle undergoes exocytosis, or there is no release. We have estimated the glutamate concentration transient at climbing fiber synapses on Purkinje cells by measuring the inhibition of excitatory postsynaptic currents (EPSCs) produced by a low-affinity competitive antagonist of AMPA receptors, γ-DGG. The results, together with simulations using a kinetic model of the AMPA receptor, suggest that the peak glutamate concentration at this synapse is dependent on release probability but is not affected by pooling of transmitter released from neighboring synapses. We propose that the mechanism responsible for the elevated glutamate concentration at this synapse is the simultaneous release of multiple vesicles per site.

Original languageEnglish (US)
Pages (from-to)301-313
Number of pages13
JournalNeuron
Volume32
Issue number2
DOIs
StatePublished - Oct 25 2001

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Purkinje Cells
Synapses
Glutamic Acid
AMPA Receptors
Synaptic Vesicles
Excitatory Postsynaptic Potentials
Exocytosis
Action Potentials

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Multivesicular release at climbing fiber-Purkinje cell synapses. / Wadiche, Jacques I.; Jahr, Craig.

In: Neuron, Vol. 32, No. 2, 25.10.2001, p. 301-313.

Research output: Contribution to journalArticle

Wadiche, Jacques I. ; Jahr, Craig. / Multivesicular release at climbing fiber-Purkinje cell synapses. In: Neuron. 2001 ; Vol. 32, No. 2. pp. 301-313.
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