Multiplex SNP discrimination

Daniel J. Fish, M. Todd Horne, Robert P. Searles, Greg P. Brewood, Albert S. Benight

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    11 Scopus citations

    Abstract

    Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.

    Original languageEnglish (US)
    Pages (from-to)L89-L91
    JournalBiophysical Journal
    Volume92
    Issue number10
    DOIs
    StatePublished - May 2007

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    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Fish, D. J., Horne, M. T., Searles, R. P., Brewood, G. P., & Benight, A. S. (2007). Multiplex SNP discrimination. Biophysical Journal, 92(10), L89-L91. https://doi.org/10.1529/biophysj.107.105320