Multiplex SNP discrimination

Daniel J. Fish, M. Todd Horne, Robert Searles, Greg P. Brewood, Albert S. Benight

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.

    Original languageEnglish (US)
    JournalBiophysical Journal
    Volume92
    Issue number10
    DOIs
    StatePublished - May 2007

    Fingerprint

    Single Nucleotide Polymorphism
    Oligonucleotide Array Sequence Analysis
    Theoretical Models

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Fish, D. J., Horne, M. T., Searles, R., Brewood, G. P., & Benight, A. S. (2007). Multiplex SNP discrimination. Biophysical Journal, 92(10). https://doi.org/10.1529/biophysj.107.105320

    Multiplex SNP discrimination. / Fish, Daniel J.; Horne, M. Todd; Searles, Robert; Brewood, Greg P.; Benight, Albert S.

    In: Biophysical Journal, Vol. 92, No. 10, 05.2007.

    Research output: Contribution to journalArticle

    Fish, DJ, Horne, MT, Searles, R, Brewood, GP & Benight, AS 2007, 'Multiplex SNP discrimination', Biophysical Journal, vol. 92, no. 10. https://doi.org/10.1529/biophysj.107.105320
    Fish, Daniel J. ; Horne, M. Todd ; Searles, Robert ; Brewood, Greg P. ; Benight, Albert S. / Multiplex SNP discrimination. In: Biophysical Journal. 2007 ; Vol. 92, No. 10.
    @article{0b17fb1f39254fc2a49ff51ad59d2b9c,
    title = "Multiplex SNP discrimination",
    abstract = "Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.",
    author = "Fish, {Daniel J.} and Horne, {M. Todd} and Robert Searles and Brewood, {Greg P.} and Benight, {Albert S.}",
    year = "2007",
    month = "5",
    doi = "10.1529/biophysj.107.105320",
    language = "English (US)",
    volume = "92",
    journal = "Biophysical Journal",
    issn = "0006-3495",
    publisher = "Biophysical Society",
    number = "10",

    }

    TY - JOUR

    T1 - Multiplex SNP discrimination

    AU - Fish, Daniel J.

    AU - Horne, M. Todd

    AU - Searles, Robert

    AU - Brewood, Greg P.

    AU - Benight, Albert S.

    PY - 2007/5

    Y1 - 2007/5

    N2 - Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.

    AB - Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.

    UR - http://www.scopus.com/inward/record.url?scp=34247899099&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=34247899099&partnerID=8YFLogxK

    U2 - 10.1529/biophysj.107.105320

    DO - 10.1529/biophysj.107.105320

    M3 - Article

    VL - 92

    JO - Biophysical Journal

    JF - Biophysical Journal

    SN - 0006-3495

    IS - 10

    ER -