Multiplex SNP discrimination

Daniel J. Fish, M. Todd Horne, Robert P. Searles, Greg P. Brewood, Albert S. Benight

    Research output: Contribution to journalArticlepeer-review

    12 Scopus citations

    Abstract

    Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.

    Original languageEnglish (US)
    Pages (from-to)L89-L91
    JournalBiophysical Journal
    Volume92
    Issue number10
    DOIs
    StatePublished - May 2007

    ASJC Scopus subject areas

    • Biophysics

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