Abstract
Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.
Original language | English (US) |
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Pages (from-to) | L89-L91 |
Journal | Biophysical Journal |
Volume | 92 |
Issue number | 10 |
DOIs | |
State | Published - May 2007 |
ASJC Scopus subject areas
- Biophysics