Multiple subunits of a voltage-dependent potassium channel contribute to the binding site for tetraethylammonium

M. P. Kavanaugh, R. S. Hurst, J. Yakel, M. D. Varnum, John Adelman, R. A. North

Research output: Contribution to journalArticle

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Abstract

RNAs encoding a wild-type (RBK1) and a mutant (RBK1(Y379V,V381 T); RBK1*) subunit of voltage-dependent potassium channels were injected into Xenopus oocytes. When expressed separately, they made homotetrameric channels that differed about 100-fold in sensitivity to tetraethylammonium (TEA). Mixtures of channels having one, two, or three low affinity subunits were expressed by injecting various proportions of RBK1 and RBK1* RNAs. The affinity for TEA of these three channel species was deduced by fitting concentration-response curves for the inhibition of potassium currents. DNAs were also concatenated to construct a sequence that encoded two connected subunits, and channels that contained four, two, or no TEA-sensitive subunits were expressed. The results suggest that bound TEA interacts simultaneously with all four subunits.

Original languageEnglish (US)
Pages (from-to)493-497
Number of pages5
JournalNeuron
Volume8
Issue number3
DOIs
StatePublished - 1992

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Tetraethylammonium
Potassium Channels
Binding Sites
RNA
Xenopus
Oocytes
Potassium
DNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kavanaugh, M. P., Hurst, R. S., Yakel, J., Varnum, M. D., Adelman, J., & North, R. A. (1992). Multiple subunits of a voltage-dependent potassium channel contribute to the binding site for tetraethylammonium. Neuron, 8(3), 493-497. https://doi.org/10.1016/0896-6273(92)90277-K

Multiple subunits of a voltage-dependent potassium channel contribute to the binding site for tetraethylammonium. / Kavanaugh, M. P.; Hurst, R. S.; Yakel, J.; Varnum, M. D.; Adelman, John; North, R. A.

In: Neuron, Vol. 8, No. 3, 1992, p. 493-497.

Research output: Contribution to journalArticle

Kavanaugh, MP, Hurst, RS, Yakel, J, Varnum, MD, Adelman, J & North, RA 1992, 'Multiple subunits of a voltage-dependent potassium channel contribute to the binding site for tetraethylammonium', Neuron, vol. 8, no. 3, pp. 493-497. https://doi.org/10.1016/0896-6273(92)90277-K
Kavanaugh, M. P. ; Hurst, R. S. ; Yakel, J. ; Varnum, M. D. ; Adelman, John ; North, R. A. / Multiple subunits of a voltage-dependent potassium channel contribute to the binding site for tetraethylammonium. In: Neuron. 1992 ; Vol. 8, No. 3. pp. 493-497.
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AU - North, R. A.

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