Multiple roles of the candidate oncogene ZNF217 in ovarian epithelial neoplastic progression

Peixiang Li, Sarah Maines-Bandiera, Wen Lin Kuo, Yinghui Guan, Yu Sun, Mark Hills, Guiqing Huang, Collin C. Collins, Peter C K Leung, Joe Gray, Nelly Auersperg

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The transcription factor ZNF217 is often amplified in ovarian cancer, but its role in neoplastic progression is unknown. We introduced ZNF217-HA by adenoviral and retroviral infection into normal human ovarian surface epithelial cells (OSE), i.e., the source of ovarian cancer, and into SV40 Tag/tag expressing, p53/ pRB-deficient OSE with extended but finite life spans (IOSE). In OSE, ZNF217-HA reduced cell-substratum adhesion and accelerated loss of senescent cells, but caused no obvious proneoplastic changes. In contrast, ZNF217-HA transduction into IOSE yielded two permanent lines, I-80RZ and I-144RZ, which exhibited telomerase activity, stable telomere lengths, anchorage independence and reduced serum dependence, but were not tumorigenic in SCID mice. This immortalization required short-term EGF treatment near the time of crisis. The permanent lines were EGF-independent, but ZNF217-dependent since siRNA to ZNF217 inhibited anchorage independence and arrested growth. Array CGH revealed genomic changes resembling those of ovarian carcinomas, such as amplicons at 3q and 20q, and deletions at 4q and 18, associated with underexpressed annexin A10, N-cadherin, desmocollin 3 and PAI-2, which have been reported as tumor suppressors. The lines overexpressed EEF1A2, SMARA3 and STAT1 and underexpressed other oncogenes, tumor suppressors and extracellular matrix/adhesion genes. The results implicate ZNF217 as an ovarian oncogene, which is detrimental to senescing normal OSE cells but contributes to neoplastic progression in OSE with inactivated p53/RB. The resemblance of the genomic changes in the ZNF217-overexpressing lines to ovarian carcinomas provides a unique model to investigate interrelationships between these changes and ovarian neoplastic phenotypes.

Original languageEnglish (US)
Pages (from-to)1863-1873
Number of pages11
JournalInternational Journal of Cancer
Volume120
Issue number9
DOIs
StatePublished - May 1 2007
Externally publishedYes

Fingerprint

Oncogenes
Epithelial Cells
Cadherins
Epidermal Growth Factor
Ovarian Neoplasms
Desmocollins
Plasminogen Activator Inhibitor 2
Carcinoma
Annexins
SCID Mice
Telomerase
antineoplaston A10
Telomere
Cell Adhesion
Small Interfering RNA
Extracellular Matrix
Neoplasms
Transcription Factors
Phenotype
Growth

Keywords

  • Immortalization
  • Oncogene
  • Ovarian cancer
  • ZNF217

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Li, P., Maines-Bandiera, S., Kuo, W. L., Guan, Y., Sun, Y., Hills, M., ... Auersperg, N. (2007). Multiple roles of the candidate oncogene ZNF217 in ovarian epithelial neoplastic progression. International Journal of Cancer, 120(9), 1863-1873. https://doi.org/10.1002/ijc.22300

Multiple roles of the candidate oncogene ZNF217 in ovarian epithelial neoplastic progression. / Li, Peixiang; Maines-Bandiera, Sarah; Kuo, Wen Lin; Guan, Yinghui; Sun, Yu; Hills, Mark; Huang, Guiqing; Collins, Collin C.; Leung, Peter C K; Gray, Joe; Auersperg, Nelly.

In: International Journal of Cancer, Vol. 120, No. 9, 01.05.2007, p. 1863-1873.

Research output: Contribution to journalArticle

Li, P, Maines-Bandiera, S, Kuo, WL, Guan, Y, Sun, Y, Hills, M, Huang, G, Collins, CC, Leung, PCK, Gray, J & Auersperg, N 2007, 'Multiple roles of the candidate oncogene ZNF217 in ovarian epithelial neoplastic progression', International Journal of Cancer, vol. 120, no. 9, pp. 1863-1873. https://doi.org/10.1002/ijc.22300
Li, Peixiang ; Maines-Bandiera, Sarah ; Kuo, Wen Lin ; Guan, Yinghui ; Sun, Yu ; Hills, Mark ; Huang, Guiqing ; Collins, Collin C. ; Leung, Peter C K ; Gray, Joe ; Auersperg, Nelly. / Multiple roles of the candidate oncogene ZNF217 in ovarian epithelial neoplastic progression. In: International Journal of Cancer. 2007 ; Vol. 120, No. 9. pp. 1863-1873.
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