Multiple fimbrial adhesins are required for full virulence of Salmonella typhimurium in mice

Adrianus W.M. Van Der Velden, Andreas J. Bäumler, Renée M. Tsolis, Fred Heffron

    Research output: Contribution to journalArticlepeer-review

    132 Scopus citations

    Abstract

    Adhesion is an important initial step during bacterial colonization of the intestinal mucosa. However, mutations in the Salmonella typhimurium fimbrial operons lpf, pef, fim only moderately alter mouse virulence. The respective adhesins may thus play only a minor role during infection or S. typhimurium may encode alternative virulence factors that can functionally compensate for their loss. To address this question, we constructed mutations in all four known fimbrial operons of S. typhimurium: fim, lpf, pef, and agf. A mutation in the agfB gene resulted in a threefold increase in the oral 50% lethal dose (LD50) of S. typhimurium for mice. In contrast, an S. typhimurium strain carrying mutations in all four fimbrial operons (quadruple mutant) had a 26-fold increased oral LD50. The quadruple mutant, but not the agfB mutant, was recovered in reduced numbers from murine fetal pellets, suggesting that a reduced ability to colonize the intestinal lumen contributed to its attenuation. These data are evidence for a synergistic action of fimbrial operons during colonization of the mouse intestine and the development of murine typhoid fever.

    Original languageEnglish (US)
    Pages (from-to)2803-2808
    Number of pages6
    JournalInfection and Immunity
    Volume66
    Issue number6
    DOIs
    StatePublished - Jun 1998

    ASJC Scopus subject areas

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

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