Multiple determinants controlling activation of yeast replication origins late in S phase

Katherine L. Friedman, John D. Diller, Betsy Ferguson, Sarah V M Nyland, Bonita J. Brewer, Walton L. Fangman

    Research output: Contribution to journalArticle

    130 Citations (Scopus)

    Abstract

    Analysis of a 131-kb segment of the left arm of yeast chromosome XIV beginning 157 kb from the telomere reveals four highly active origins of replication that initiate replication late in S phase. Previous work has shown that telomeres act as determinants for late origin activation. However, at least two of the chromosome XIV origins maintain their late activation time when located on large circular plasmids, indicating that late replication is independent of telomeres. Analysis of the replication time of plasmid derivatives containing varying amounts of chromosome XIV DNA show that a minimum of three chromosomal elements, distinct from each tested origin, contribute to late activation time. These late determinants are functionally equivalent, because duplication of one set of contributing sequences can compensate for the removal of another set. Furthermore, insertion of an origin that is normally early activated into this domain results in a shift to late activation, suggesting that the chromosome XIV origins are not unique in their ability to respond to the late determinants.

    Original languageEnglish (US)
    Pages (from-to)1595-1607
    Number of pages13
    JournalGenes and Development
    Volume10
    Issue number13
    StatePublished - 1996

    Fingerprint

    Replication Origin
    S Phase
    Telomere
    Chromosomes
    Yeasts
    Plasmids
    DNA

    Keywords

    • 2D agarose gels
    • Chromosomal domains
    • chromosome walk
    • Saccharomyces cerevisiae

    ASJC Scopus subject areas

    • Genetics
    • Developmental Biology

    Cite this

    Friedman, K. L., Diller, J. D., Ferguson, B., Nyland, S. V. M., Brewer, B. J., & Fangman, W. L. (1996). Multiple determinants controlling activation of yeast replication origins late in S phase. Genes and Development, 10(13), 1595-1607.

    Multiple determinants controlling activation of yeast replication origins late in S phase. / Friedman, Katherine L.; Diller, John D.; Ferguson, Betsy; Nyland, Sarah V M; Brewer, Bonita J.; Fangman, Walton L.

    In: Genes and Development, Vol. 10, No. 13, 1996, p. 1595-1607.

    Research output: Contribution to journalArticle

    Friedman, KL, Diller, JD, Ferguson, B, Nyland, SVM, Brewer, BJ & Fangman, WL 1996, 'Multiple determinants controlling activation of yeast replication origins late in S phase', Genes and Development, vol. 10, no. 13, pp. 1595-1607.
    Friedman KL, Diller JD, Ferguson B, Nyland SVM, Brewer BJ, Fangman WL. Multiple determinants controlling activation of yeast replication origins late in S phase. Genes and Development. 1996;10(13):1595-1607.
    Friedman, Katherine L. ; Diller, John D. ; Ferguson, Betsy ; Nyland, Sarah V M ; Brewer, Bonita J. ; Fangman, Walton L. / Multiple determinants controlling activation of yeast replication origins late in S phase. In: Genes and Development. 1996 ; Vol. 10, No. 13. pp. 1595-1607.
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    AU - Fangman, Walton L.

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