Multiple control elments for the uvrC gene unit of Escherichia coli

Surendra Shanna, Thomas F. Stark, Wanda G. Beattie, Robb Moses

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We have sequenced the control region of the uvrC protein Including two open reading frames (ORF) encoding polypeptides of 28 kd and 23 fed molecular weight. The uvrC gene is preceded by five promoters. The P1, P2a and P2b promoter sequences are 5' to the 28 kd and the 23 kd proteins respectively. The P3 and P4 promoters are located within the structural gene for the 23 kd protein. The P3 promoter is required for adequate in vivo expression. There are three putative lexA protein binding sites, detected at the 3' end of the 28 kd protein (lexA1), within the coding sequences for the 23 kd protein (lexA2) and within the P3 promoter (lexA3). Promoter P2 is responsible for transcription of the uvrC gene, producing transcripts of 2.8 and 1.6 kb. The upstream region including the 28 kd protein is required for enhanced expression under non-induced conditions. These results show that the uvrC gene is controlled by multiple promoters and is transcribed as part of a multigene unit.

Original languageEnglish (US)
Pages (from-to)2301-2318
Number of pages18
JournalNucleic Acids Research
Volume14
Issue number5
DOIs
StatePublished - Mar 11 1986
Externally publishedYes

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Promoter
Escherichia coli
Escherichia Coli
Genes
Gene
Proteins
Protein
Unit
Polypeptides
Binding sites
Transcription
Protein Binding
Open Reading Frames
Molecular Weight
Molecular weight
Binding Sites
Encoding
Coding
Peptides

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Multiple control elments for the uvrC gene unit of Escherichia coli. / Shanna, Surendra; Stark, Thomas F.; Beattie, Wanda G.; Moses, Robb.

In: Nucleic Acids Research, Vol. 14, No. 5, 11.03.1986, p. 2301-2318.

Research output: Contribution to journalArticle

Shanna, Surendra ; Stark, Thomas F. ; Beattie, Wanda G. ; Moses, Robb. / Multiple control elments for the uvrC gene unit of Escherichia coli. In: Nucleic Acids Research. 1986 ; Vol. 14, No. 5. pp. 2301-2318.
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