Multiple conductance classes of mouse nicotinic acetylcholine receptors expressed in Xenopus oocytes

R. Kullberg, J. L. Owens, P. Camacho, Gail Mandel, Paul Brehm

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Acetylcholine receptor (AcChoR) subunit mRNAs transcribed from mouse BC3H-1 cDNAs were injected into Xenopus oocytes and the expressed AcChoR channels were examined by single channel recording. Injection of α-, β-, γ-, and δ-subunit mRNAs produced two predominant channel classes with conductances of ≈50 and ≈12 pS, while infrequent openings of ≈25-pS channels were also observed. Injection of α-, β-, and γ-subunit mRNAs produced a single class of ≈12-pS AcChoR channels, which resembled the smallest conductance channels present in αβγδ-injected oocytes. Assembly of δ-less channels may thus explain the lowest conductance AcChoR channels in αβγδ-injected oocytes and might also account for similar channels that have been observed in vertebrate skeletal muscle.

Original languageEnglish (US)
Pages (from-to)2067-2071
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number6
StatePublished - Mar 1990
Externally publishedYes

Fingerprint

Nicotinic Receptors
Cholinergic Receptors
Xenopus
Oocytes
Messenger RNA
Injections
Vertebrates
Skeletal Muscle
Complementary DNA

Keywords

  • Receptor assembly
  • Single channel recording

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Multiple conductance classes of mouse nicotinic acetylcholine receptors expressed in Xenopus oocytes. / Kullberg, R.; Owens, J. L.; Camacho, P.; Mandel, Gail; Brehm, Paul.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 6, 03.1990, p. 2067-2071.

Research output: Contribution to journalArticle

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