TY - JOUR
T1 - Multiple conductance classes of mouse nicotinic acetylcholine receptors expressed in Xenopus oocytes
AU - Kullberg, R.
AU - Owens, J. L.
AU - Camacho, P.
AU - Mandel, G.
AU - Brehm, P.
PY - 1990/3
Y1 - 1990/3
N2 - Acetylcholine receptor (AcChoR) subunit mRNAs transcribed from mouse BC3H-1 cDNAs were injected into Xenopus oocytes and the expressed AcChoR channels were examined by single channel recording. Injection of α-, β-, γ-, and δ-subunit mRNAs produced two predominant channel classes with conductances of ≈50 and ≈12 pS, while infrequent openings of ≈25-pS channels were also observed. Injection of α-, β-, and γ-subunit mRNAs produced a single class of ≈12-pS AcChoR channels, which resembled the smallest conductance channels present in αβγδ-injected oocytes. Assembly of δ-less channels may thus explain the lowest conductance AcChoR channels in αβγδ-injected oocytes and might also account for similar channels that have been observed in vertebrate skeletal muscle.
AB - Acetylcholine receptor (AcChoR) subunit mRNAs transcribed from mouse BC3H-1 cDNAs were injected into Xenopus oocytes and the expressed AcChoR channels were examined by single channel recording. Injection of α-, β-, γ-, and δ-subunit mRNAs produced two predominant channel classes with conductances of ≈50 and ≈12 pS, while infrequent openings of ≈25-pS channels were also observed. Injection of α-, β-, and γ-subunit mRNAs produced a single class of ≈12-pS AcChoR channels, which resembled the smallest conductance channels present in αβγδ-injected oocytes. Assembly of δ-less channels may thus explain the lowest conductance AcChoR channels in αβγδ-injected oocytes and might also account for similar channels that have been observed in vertebrate skeletal muscle.
KW - Receptor assembly
KW - Single channel recording
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U2 - 10.1073/pnas.87.6.2067
DO - 10.1073/pnas.87.6.2067
M3 - Article
C2 - 2315303
AN - SCOPUS:0025246297
SN - 0027-8424
VL - 87
SP - 2067
EP - 2071
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -