TY - JOUR
T1 - Multiple antibiotic susceptibility associated with inactivation of the prc gene
AU - Seoane, A.
AU - Sabbaj, A.
AU - McMurry, L. M.
AU - Levy, S. B.
PY - 1992
Y1 - 1992
N2 - A Tn5 insertion which led to increased susceptibility to multiple drugs, including tetracycline, chloramphenicol, nalidixic acid, erythromycin, spectinomycin, norfloxacin, and novobiocin, was identified in Escherichia coli. Cloning and sequence studies showed that the insertion was in the previously identified prc gene at min 40.4. The prc product is known to function as a protease linked to processing of penicillin-binding protein 3 and λ repressor and when absent to allow some leakage of periplasmic constituents. Complementation studies with the prc gene on plasmids showed complete recovery of parental levels of susceptibility to all drugs except chloramphenicol, with which only partial reversion to wild-type levels was observed.
AB - A Tn5 insertion which led to increased susceptibility to multiple drugs, including tetracycline, chloramphenicol, nalidixic acid, erythromycin, spectinomycin, norfloxacin, and novobiocin, was identified in Escherichia coli. Cloning and sequence studies showed that the insertion was in the previously identified prc gene at min 40.4. The prc product is known to function as a protease linked to processing of penicillin-binding protein 3 and λ repressor and when absent to allow some leakage of periplasmic constituents. Complementation studies with the prc gene on plasmids showed complete recovery of parental levels of susceptibility to all drugs except chloramphenicol, with which only partial reversion to wild-type levels was observed.
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U2 - 10.1128/jb.174.23.7844-7847.1992
DO - 10.1128/jb.174.23.7844-7847.1992
M3 - Comment/debate
C2 - 1447154
AN - SCOPUS:0026457932
SN - 0021-9193
VL - 174
SP - 7844
EP - 7847
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 23
ER -