Multiple aberrant splicing of the p53 transcript without genomic mutations around exon-intron junctions in a case of chronic myelogenous leukaemia in blast crisis: A possible novel mechanism of p53 inactivation

Hiroyuki Nakai, H. Kaneko, S. Horiike, Y. Ariyama, S. Misawa, K. Kashima, K. Ishizaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We found three truncated p53 transcripts in a patient with chronic myelogenous leukaemia in blast crisis carrying chromosome 17 abnormalities. Sequencing of these transcripts revealed complete absence of the entire exons 7, 8 and 9 in one, exons 8 and 9 in another, and exon 10 in the other. Sequencing analysis of genomic DNA, however, revealed no mutation in exons 6-10 and their flanking introns. These results suggest that the aberrarnt p53 transcripts in this case might not result from splicing mutations but from an unknown affected splicing process.

Original languageEnglish (US)
Pages (from-to)839-842
Number of pages4
JournalBritish Journal of Haematology
Volume87
Issue number4
StatePublished - 1994
Externally publishedYes

Fingerprint

Blast Crisis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Introns
Exons
Mutation
Chromosomes, Human, Pair 17
Chromosome Aberrations
DNA

Keywords

  • Aberrant splicing
  • Chromosome 17
  • CML
  • p53
  • Splicing mutation

ASJC Scopus subject areas

  • Hematology

Cite this

Multiple aberrant splicing of the p53 transcript without genomic mutations around exon-intron junctions in a case of chronic myelogenous leukaemia in blast crisis : A possible novel mechanism of p53 inactivation. / Nakai, Hiroyuki; Kaneko, H.; Horiike, S.; Ariyama, Y.; Misawa, S.; Kashima, K.; Ishizaki, K.

In: British Journal of Haematology, Vol. 87, No. 4, 1994, p. 839-842.

Research output: Contribution to journalArticle

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AU - Kaneko, H.

AU - Horiike, S.

AU - Ariyama, Y.

AU - Misawa, S.

AU - Kashima, K.

AU - Ishizaki, K.

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KW - Splicing mutation

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