Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe

Gangjun Liu, Khanh Kieu, Frank W. Wise, Zhongping Chen

Research output: Contribution to journalLetter

40 Scopus citations


We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.

Original languageEnglish (US)
Pages (from-to)34-39
Number of pages6
JournalJournal of Biophotonics
Issue number1-2
StatePublished - Jan 1 2011



  • Fiber laser
  • Hand-held probe
  • Multiphoton processes
  • Nonlinear microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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