Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe

Gangjun Liu, Khanh Kieu, Frank W. Wise, Zhongping Chen

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.

Original languageEnglish (US)
Pages (from-to)34-39
Number of pages6
JournalJournal of Biophotonics
Volume4
Issue number1-2
DOIs
StatePublished - Jan 2011
Externally publishedYes

Fingerprint

Ultrashort pulses
Photons
Lenses
Microscopy
Microscopic examination
Lasers
Optics and Photonics
microscopy
fiber lasers
fibers
Fibers
probes
pulses
lenses
Galvanometers
lasers
galvanometers
Photonic crystal fibers
Hand
Fluorescence

Keywords

  • Fiber laser
  • Hand-held probe
  • Multiphoton processes
  • Nonlinear microscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe. / Liu, Gangjun; Kieu, Khanh; Wise, Frank W.; Chen, Zhongping.

In: Journal of Biophotonics, Vol. 4, No. 1-2, 01.2011, p. 34-39.

Research output: Contribution to journalArticle

Liu, Gangjun ; Kieu, Khanh ; Wise, Frank W. ; Chen, Zhongping. / Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe. In: Journal of Biophotonics. 2011 ; Vol. 4, No. 1-2. pp. 34-39.
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