Multiparameter imaging for the analysis of intracellular signaling

Carsten Schultz, Andreas Schleifenbaum, Joachim Goedhart, Theodorus W.J. Gadella

Research output: Contribution to journalReview article

36 Scopus citations

Abstract

In biological experimentation and especially in drug discovery there is a trend towards more complex test systems. Cell-based assays are replacing conventional binding or enzyme assays more and more. This development is strongly driven by novel fluorescent probes that give insight into cellular processes. Target proteins are studied in their natural environment; this gives much more realistic test results, especially with respect to enzyme location and kinetics. However, in the complex environment of cells, many parameters contribute to the performance of the protein of interest. Therefore, it would be desirable to monitor simultaneously as many of the relevant cellular processes as possible. Here, we discuss the possibilities and limitations provided by multiparameter monitoring of cellular events with fluorescent probes. Some novel examples of the use of fluorescent probes and multiparameter imaging are shown.

Original languageEnglish (US)
Pages (from-to)1323-1330
Number of pages8
JournalChemBioChem
Volume6
Issue number8
DOIs
StatePublished - Aug 1 2005

Keywords

  • Cell signaling
  • FRET
  • Fluorescence microscopy
  • Fluorescent probes
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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    Schultz, C., Schleifenbaum, A., Goedhart, J., & Gadella, T. W. J. (2005). Multiparameter imaging for the analysis of intracellular signaling. ChemBioChem, 6(8), 1323-1330. https://doi.org/10.1002/cbic.200500012