Multinucleated variant endothelial cells (MVECs) of human aorta: Expression of tumor suppressor gene p53 and relationship to atherosclerosis and aging

Toshimi Satoh, Eizaburo Sasatomi, Fumio Yamasaki, Hironori Ishida, Liqing Wu, Osamu Tokunaga

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Multinucleated variant endothelial cells (MVECs) generally exist in atherosclerotic human aorta and even in nonatherosclerotic aorta. Because the number of nuclei is increased in every MVEC, and because DNA instability was suspected, a series of oncogene expressions was conducted to clarify the nature of nuclear abnormality. The tumor suppressor gene p53 was found to be specifically expressed in the multinuclei of MVECs, while double nuclei were sometimes positive, and mononuclear typical endothelial cells were always negative for p53. Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) revealed extra bands in exons 5 and 7 of the p53 gene, but no additional band in exons 6 and 8. In a BCL familiy, BCL-2 was coexpressed in one or two nuclei in the perinuclear space of the multinuclei of MVECs, whereas MCL-1, BCL-XS/L, and BAX were all negative, indicating that the BCL-2 coding gene is expressed only in the corresponding one or two nuclei of the multinuclei. Another oncogene, c-MET (hepatocyte growth factor receptor), was universally expressed in either type of endothelial cells, but other oncogenes, k-RAS and C-ERBB2, were not expressed in either type. MVECs were derived from human aorta and therefore non-tumorous somatic cells. No morphologic evidence of apoptosis was found. Although it is unclear that the extra bands came from the MVECs or just from ECs associated with atherosclerosis, combined immunocytological studies and PCR analysis suggest that MVECs express mutant type p53.

Original languageEnglish (US)
Pages (from-to)123-132
Number of pages10
JournalEndothelium: Journal of Endothelial Cell Research
Volume6
Issue number2
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Apoptosis
  • Endothelium
  • Gene
  • Mutation
  • Variant
  • p53

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Multinucleated variant endothelial cells (MVECs) of human aorta: Expression of tumor suppressor gene p53 and relationship to atherosclerosis and aging'. Together they form a unique fingerprint.

Cite this