Multinuclear NMR studies of an actively dividing artificial tumor

E. G. Shankland, J. C. Livesey, R. W. Wiseman, Kenneth Krohn

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Growth of the A549 cell line in a perfusion system suitable for use in a magnetic resonance study has been characterized and shown to be stable physiologically and hence appropriate for serial observations. Several methods of monitoring cell growth were compared to assess the behavior of the cells in this system. Comparison between NMR metabolite data and cell growth via cell counting showed that 31 P NMR signals accurately reported cell doubling time. In contrast to most NMR cell culture systems, viable cells can be recovered from the perfusion system after the NMR measurements for further biochemical studies. These data further suggest that this system will be useful for studying the physiology and biochemistry of exponentially growing cells for at least two days in NMR tube culture.

Original languageEnglish (US)
Pages (from-to)49-58
Number of pages10
JournalPhysiological Research
Volume51
Issue number1
StatePublished - 2002
Externally publishedYes

Fingerprint

Biomolecular Nuclear Magnetic Resonance
Neoplasms
Growth
Perfusion
Biochemistry
Magnetic Resonance Spectroscopy
Cell Culture Techniques

Keywords

  • A549
  • Cell culture
  • NMR
  • Phophorous

ASJC Scopus subject areas

  • Physiology

Cite this

Shankland, E. G., Livesey, J. C., Wiseman, R. W., & Krohn, K. (2002). Multinuclear NMR studies of an actively dividing artificial tumor. Physiological Research, 51(1), 49-58.

Multinuclear NMR studies of an actively dividing artificial tumor. / Shankland, E. G.; Livesey, J. C.; Wiseman, R. W.; Krohn, Kenneth.

In: Physiological Research, Vol. 51, No. 1, 2002, p. 49-58.

Research output: Contribution to journalArticle

Shankland, EG, Livesey, JC, Wiseman, RW & Krohn, K 2002, 'Multinuclear NMR studies of an actively dividing artificial tumor', Physiological Research, vol. 51, no. 1, pp. 49-58.
Shankland, E. G. ; Livesey, J. C. ; Wiseman, R. W. ; Krohn, Kenneth. / Multinuclear NMR studies of an actively dividing artificial tumor. In: Physiological Research. 2002 ; Vol. 51, No. 1. pp. 49-58.
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