Multimodal confocal mosaicing microscopy: An emphasis on squamous cell carcinoma

Nathaniel W. Chen, Jordan Sensibaugh, Ardaland Ardeshiri, Adam Blanchard, Steven Jacques, Daniel Gareau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Our previous study reported a sensitivity of 96.6% and a specificity of 89.2% in rapidly detecting Basal Cell Carcinomas (BCCs) when nuclei were stained with acridine orange. Squamous Cell Carcinomas (SCCs) and infiltrative BCCs remain difficult to detect. More complete screening can be achieved utilizing both acridine orange for nuclei staining and eosin for cytoplasmic contrast, using two lasers to excite the two stains independently. Nuclear fluorescence is achieved by staining with acridine orange (0.5mM, 60 s), and cytoplasmic fluorescence is achieved by staining with eosin working solution (30 s). This work shows good morphological contrast of SCC and infiltrative BCC with eosin, acridine orange, and reflectance, and presents a means for rapid SCC and infiltrative BCC detection in fresh skin excisions using multimodal confocal microscopy. In addition, digital staining is shown to effectively simulate hematoxylin and eosin (H&E) histology with confocal mosaics.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7548
DOIs
StatePublished - 2010
EventPhotonic Therapeutics and Diagnostics VI - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Other

OtherPhotonic Therapeutics and Diagnostics VI
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Fingerprint

Acridine Orange
Confocal microscopy
Basal Cell Carcinoma
Eosine Yellowish-(YS)
Confocal Microscopy
Squamous Cell Carcinoma
cancer
Staining and Labeling
microscopy
staining
Fluorescence
Histology
Skin
Screening
Hematoxylin
Cells
Lasers
Coloring Agents
fluorescence
nuclei

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Chen, N. W., Sensibaugh, J., Ardeshiri, A., Blanchard, A., Jacques, S., & Gareau, D. (2010). Multimodal confocal mosaicing microscopy: An emphasis on squamous cell carcinoma. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7548). [75480K] https://doi.org/10.1117/12.842746

Multimodal confocal mosaicing microscopy : An emphasis on squamous cell carcinoma. / Chen, Nathaniel W.; Sensibaugh, Jordan; Ardeshiri, Ardaland; Blanchard, Adam; Jacques, Steven; Gareau, Daniel.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7548 2010. 75480K.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chen, NW, Sensibaugh, J, Ardeshiri, A, Blanchard, A, Jacques, S & Gareau, D 2010, Multimodal confocal mosaicing microscopy: An emphasis on squamous cell carcinoma. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7548, 75480K, Photonic Therapeutics and Diagnostics VI, San Francisco, CA, United States, 1/23/10. https://doi.org/10.1117/12.842746
Chen NW, Sensibaugh J, Ardeshiri A, Blanchard A, Jacques S, Gareau D. Multimodal confocal mosaicing microscopy: An emphasis on squamous cell carcinoma. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7548. 2010. 75480K https://doi.org/10.1117/12.842746
Chen, Nathaniel W. ; Sensibaugh, Jordan ; Ardeshiri, Ardaland ; Blanchard, Adam ; Jacques, Steven ; Gareau, Daniel. / Multimodal confocal mosaicing microscopy : An emphasis on squamous cell carcinoma. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7548 2010.
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