TY - GEN
T1 - Multimodal confocal mosaicing microscopy
T2 - Photonic Therapeutics and Diagnostics VI
AU - Chen, Nathaniel W.
AU - Sensibaugh, Jordan
AU - Ardeshiri, Ardaland
AU - Blanchard, Adam
AU - Jacques, Steven
AU - Gareau, Daniel
PY - 2010
Y1 - 2010
N2 - Our previous study reported a sensitivity of 96.6% and a specificity of 89.2% in rapidly detecting Basal Cell Carcinomas (BCCs) when nuclei were stained with acridine orange. Squamous Cell Carcinomas (SCCs) and infiltrative BCCs remain difficult to detect. More complete screening can be achieved utilizing both acridine orange for nuclei staining and eosin for cytoplasmic contrast, using two lasers to excite the two stains independently. Nuclear fluorescence is achieved by staining with acridine orange (0.5mM, 60 s), and cytoplasmic fluorescence is achieved by staining with eosin working solution (30 s). This work shows good morphological contrast of SCC and infiltrative BCC with eosin, acridine orange, and reflectance, and presents a means for rapid SCC and infiltrative BCC detection in fresh skin excisions using multimodal confocal microscopy. In addition, digital staining is shown to effectively simulate hematoxylin and eosin (H&E) histology with confocal mosaics.
AB - Our previous study reported a sensitivity of 96.6% and a specificity of 89.2% in rapidly detecting Basal Cell Carcinomas (BCCs) when nuclei were stained with acridine orange. Squamous Cell Carcinomas (SCCs) and infiltrative BCCs remain difficult to detect. More complete screening can be achieved utilizing both acridine orange for nuclei staining and eosin for cytoplasmic contrast, using two lasers to excite the two stains independently. Nuclear fluorescence is achieved by staining with acridine orange (0.5mM, 60 s), and cytoplasmic fluorescence is achieved by staining with eosin working solution (30 s). This work shows good morphological contrast of SCC and infiltrative BCC with eosin, acridine orange, and reflectance, and presents a means for rapid SCC and infiltrative BCC detection in fresh skin excisions using multimodal confocal microscopy. In addition, digital staining is shown to effectively simulate hematoxylin and eosin (H&E) histology with confocal mosaics.
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U2 - 10.1117/12.842746
DO - 10.1117/12.842746
M3 - Conference contribution
AN - SCOPUS:79751468959
SN - 9780819479440
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photonic Therapeutics and Diagnostics VI
Y2 - 23 January 2010 through 25 January 2010
ER -