TY - GEN
T1 - Multilayer BioDerivatized nanoparticle composites by electric field directed self-assembly
AU - Heller, Michael J.
AU - Haiso, Alexander P.
PY - 2010
Y1 - 2010
N2 - An electronic microarray has been used to carry out directed self-assembly of higher order 3D structures from biotin/streptavidin, DNA and enzyme derivatized nanoparticles. Structures with up to fifty layers of alternating biotin and streptavidin, DNA and enzyme nanoparticles were fabricated using a 400 site CMOS microarray system. In this process, reconfigurable electric fields produced by the microarray were used to rapidly transport, concentrate and accelerate the binding of 40 and/or 200nm nanometer bio-derivatized nanoparticles to selected sites on the microarray. The nanoparticle layering process takes less than one minute per layer. The nanoparticle addressing/binding process was monitored by changes in fluorescence intensity as each nanoparticle layer was deposited. The final multilayered 3-D structures are about two microns in thickness and 50 microns in diameter. Most recently we have successfully fabricated enzyme-nanoparticle layers with streptavidin-alkaline phosphatase, glucose oxidase-avidin, and streptavidin-HRP. Up to 47 layers were addressed with 200nm nanoparticles and enzyme activity was retained in the assembled structure. This work represents a unique example of combining "top-down" and "bottom-up" technologies into a novel nanofabrication process. Such a process will be useful for the hierarchal assembly of 3D nano, micro, and macrostructures for a variety of electronic/photonic, nanomaterials, energy (photovoltaics, fuel cells, batteries) and biosensor applications.
AB - An electronic microarray has been used to carry out directed self-assembly of higher order 3D structures from biotin/streptavidin, DNA and enzyme derivatized nanoparticles. Structures with up to fifty layers of alternating biotin and streptavidin, DNA and enzyme nanoparticles were fabricated using a 400 site CMOS microarray system. In this process, reconfigurable electric fields produced by the microarray were used to rapidly transport, concentrate and accelerate the binding of 40 and/or 200nm nanometer bio-derivatized nanoparticles to selected sites on the microarray. The nanoparticle layering process takes less than one minute per layer. The nanoparticle addressing/binding process was monitored by changes in fluorescence intensity as each nanoparticle layer was deposited. The final multilayered 3-D structures are about two microns in thickness and 50 microns in diameter. Most recently we have successfully fabricated enzyme-nanoparticle layers with streptavidin-alkaline phosphatase, glucose oxidase-avidin, and streptavidin-HRP. Up to 47 layers were addressed with 200nm nanoparticles and enzyme activity was retained in the assembled structure. This work represents a unique example of combining "top-down" and "bottom-up" technologies into a novel nanofabrication process. Such a process will be useful for the hierarchal assembly of 3D nano, micro, and macrostructures for a variety of electronic/photonic, nanomaterials, energy (photovoltaics, fuel cells, batteries) and biosensor applications.
KW - Composites
KW - Electric field
KW - Higher order structures
KW - Nanofabrication
KW - Nanoparticles
KW - Self-assembly
UR - http://www.scopus.com/inward/record.url?scp=78049431512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78049431512&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:78049431512
SN - 9781439834152
T3 - Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
SP - 250
EP - 253
BT - Nanotechnology 2010
T2 - Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Y2 - 21 June 2010 through 24 June 2010
ER -