Multigene DNA prime-boost vaccines for SHIV89.6P

Nicole A. Doria-Rose, Christopher C. Pierce, Michael T. Hensel, William F. Sutton, Nadeem Sheikh, Patricia Polacino, La Rene Kuller, Yong De Zhu, Shiu Lok Hu, David Anderson, Nancy L. Haigwood

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    15 Scopus citations

    Abstract

    We assessed four prime-boost vaccine regimens with a Gene Gun component for SHIV89.6P in Macaca nemestrina. A dosing experiment using β-galactosidase plasmid showed that 30 or 45 shots per dose elicited higher titer antibody than smaller doses. For SHIV89.6P, we administered a six-plasmid vaccine capable of producing non-infectious virions in vivo in combination with either vaccinia recombinants or inactivated virus. DNA prime/vaccinia boost, or the reverse, elicited strong immune responses. The SHIV89.6P challenge virus was grown in M. nemestrina peripheral blood mononuclear cells and titered in vivo intrarectally. As has been observed for SHIV89.6P in M. mulatta, the infected M. nemestrina experienced rapid and severe loss of circulating CD4+ T cells. Vaccinated macaques were challenged three weeks after the last boost. DNA prime/vaccina boost or vaccina prime/DNA boost protected 11/12 animals from acute CD4+ T cell depletion and disease, while other regimens were not effective.

    Original languageEnglish (US)
    Pages (from-to)218-228
    Number of pages11
    JournalJournal of medical primatology
    Volume32
    Issue number4-5
    DOIs
    StatePublished - Aug 1 2003

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    Keywords

    • Dose
    • Gene gun
    • Prime-boost
    • SHIV89.6P
    • Vaccine

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • veterinary(all)

    Cite this

    Doria-Rose, N. A., Pierce, C. C., Hensel, M. T., Sutton, W. F., Sheikh, N., Polacino, P., Kuller, L. R., Zhu, Y. D., Hu, S. L., Anderson, D., & Haigwood, N. L. (2003). Multigene DNA prime-boost vaccines for SHIV89.6P. Journal of medical primatology, 32(4-5), 218-228. https://doi.org/10.1034/j.1600-0684.2003.00028.x