Multigene DNA prime-boost vaccines for SHIV89.6P

Nicole A. Doria-Rose, Christopher C. Pierce, Michael T. Hensel, William F. Sutton, Nadeem Sheikh, Patricia Polacino, LaRene Kuller, Yong De Zhu, Shiu Lok Hu, David Anderson, Nancy Haigwood

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We assessed four prime-boost vaccine regimens with a Gene Gun component for SHIV89.6P in Macaca nemestrina. A dosing experiment using β-galactosidase plasmid showed that 30 or 45 shots per dose elicited higher titer antibody than smaller doses. For SHIV89.6P, we administered a six-plasmid vaccine capable of producing non-infectious virions in vivo in combination with either vaccinia recombinants or inactivated virus. DNA prime/vaccinia boost, or the reverse, elicited strong immune responses. The SHIV89.6P challenge virus was grown in M. nemestrina peripheral blood mononuclear cells and titered in vivo intrarectally. As has been observed for SHIV89.6P in M. mulatta, the infected M. nemestrina experienced rapid and severe loss of circulating CD4+ T cells. Vaccinated macaques were challenged three weeks after the last boost. DNA prime/vaccina boost or vaccina prime/DNA boost protected 11/12 animals from acute CD4+ T cell depletion and disease, while other regimens were not effective.

Original languageEnglish (US)
Pages (from-to)218-228
Number of pages11
JournalJournal of Medical Primatology
Volume32
Issue number4-5
DOIs
StatePublished - Aug 2003
Externally publishedYes

Fingerprint

Macaca nemestrina
Vaccinia
Vaccines
Vaccinium
vaccines
DNA
plasmids
Plasmids
T-lymphocytes
Galactosidases
Viruses
Gene Components
T-Lymphocytes
galactosidases
viruses
Firearms
Macaca
mononuclear leukocytes
dosage
virion

Keywords

  • Dose
  • Gene gun
  • Prime-boost
  • SHIV89.6P
  • Vaccine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Doria-Rose, N. A., Pierce, C. C., Hensel, M. T., Sutton, W. F., Sheikh, N., Polacino, P., ... Haigwood, N. (2003). Multigene DNA prime-boost vaccines for SHIV89.6P. Journal of Medical Primatology, 32(4-5), 218-228. https://doi.org/10.1034/j.1600-0684.2003.00028.x

Multigene DNA prime-boost vaccines for SHIV89.6P. / Doria-Rose, Nicole A.; Pierce, Christopher C.; Hensel, Michael T.; Sutton, William F.; Sheikh, Nadeem; Polacino, Patricia; Kuller, LaRene; Zhu, Yong De; Hu, Shiu Lok; Anderson, David; Haigwood, Nancy.

In: Journal of Medical Primatology, Vol. 32, No. 4-5, 08.2003, p. 218-228.

Research output: Contribution to journalArticle

Doria-Rose, NA, Pierce, CC, Hensel, MT, Sutton, WF, Sheikh, N, Polacino, P, Kuller, L, Zhu, YD, Hu, SL, Anderson, D & Haigwood, N 2003, 'Multigene DNA prime-boost vaccines for SHIV89.6P', Journal of Medical Primatology, vol. 32, no. 4-5, pp. 218-228. https://doi.org/10.1034/j.1600-0684.2003.00028.x
Doria-Rose NA, Pierce CC, Hensel MT, Sutton WF, Sheikh N, Polacino P et al. Multigene DNA prime-boost vaccines for SHIV89.6P. Journal of Medical Primatology. 2003 Aug;32(4-5):218-228. https://doi.org/10.1034/j.1600-0684.2003.00028.x
Doria-Rose, Nicole A. ; Pierce, Christopher C. ; Hensel, Michael T. ; Sutton, William F. ; Sheikh, Nadeem ; Polacino, Patricia ; Kuller, LaRene ; Zhu, Yong De ; Hu, Shiu Lok ; Anderson, David ; Haigwood, Nancy. / Multigene DNA prime-boost vaccines for SHIV89.6P. In: Journal of Medical Primatology. 2003 ; Vol. 32, No. 4-5. pp. 218-228.
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