Multicolor lineage tracing using in vivo time-lapse imaging reveals coordinated death of clonally related cells in the developing vertebrate brain

Nicole L. Brockway, Zoe T. Cook, Maritte J. O'Gallagher, Zachary J.C. Tobias, Mako Gedi, Kristine M. Carey, Vivek Unni, Y. Albert Pan, Margaret R. Metz, Tamily A. Weissman

Research output: Contribution to journalArticle

Abstract

The global mechanisms that regulate and potentially coordinate cell proliferation & death in developing neural regions are not well understood. In particular, it is not clear how or whether clonal relationships between neural progenitor cells and their progeny influence the growing brain. We have developed an approach using Brainbow in the developing zebrafish to visualize and follow multiple clones of related cells in vivo over time. This allows for clear visualization of many dividing clones of cells, deep in proliferating brain regions. As expected, in addition to undergoing interkinetic nuclear migration and cell division, cells also periodically undergo apoptosis. Interestingly, cell death occurs in a non-random manner: clonally related cells are more likely to die in a progressive fashion than cells from different clones. Multiple members of an individual clone die while neighboring clones appear healthy and continue to divide. Our results suggest that clonal relationships can influence cellular fitness and survival in the developing nervous system, perhaps through a competitive mechanism whereby clones of cells are competing with other clones. Clonal cell competition may help regulate neuronal proliferation in the vertebrate brain.

Original languageEnglish (US)
JournalDevelopmental Biology
DOIs
StatePublished - Jan 1 2019

Fingerprint

Time-Lapse Imaging
Vertebrates
Clone Cells
Brain
Cell Nucleus Division
Zebrafish
Cell Division
Nervous System
Cell Death
Stem Cells
Cell Proliferation
Apoptosis

Keywords

  • Apoptosis
  • Brainbow
  • Competition
  • Development
  • Hindbrain
  • In vivo imaging
  • Nervous system
  • Zebrabow
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Multicolor lineage tracing using in vivo time-lapse imaging reveals coordinated death of clonally related cells in the developing vertebrate brain. / Brockway, Nicole L.; Cook, Zoe T.; O'Gallagher, Maritte J.; Tobias, Zachary J.C.; Gedi, Mako; Carey, Kristine M.; Unni, Vivek; Pan, Y. Albert; Metz, Margaret R.; Weissman, Tamily A.

In: Developmental Biology, 01.01.2019.

Research output: Contribution to journalArticle

Brockway, Nicole L. ; Cook, Zoe T. ; O'Gallagher, Maritte J. ; Tobias, Zachary J.C. ; Gedi, Mako ; Carey, Kristine M. ; Unni, Vivek ; Pan, Y. Albert ; Metz, Margaret R. ; Weissman, Tamily A. / Multicolor lineage tracing using in vivo time-lapse imaging reveals coordinated death of clonally related cells in the developing vertebrate brain. In: Developmental Biology. 2019.
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