@article{f555ed1d2ca34024903d8a14824cef1a,
title = "Multicolor labeling of airway neurons and analysis of parasympathetic heterogeneity",
abstract = "We report subpopulations of airway parasympathetic neurons expressing substance P, neuronal nitric oxide synthase, and tyrosine hydroxylase, highlighting unexplored heterogeneity in this population. These neurotransmitter-specific subpopulations did not form intraganglionic interneurons, but rather, extended outside the ganglia, into the airways, to distant innervation targets. Our experiments demonstrate the utility of multicolor labeling to characterize airway innervation, allowing us to confirm the extensive heterogeneity of postganglionic parasympathetic neurons. These methods will facilitate future investigations of neurophysiology and neural contributions to airway disease.",
author = "Pincus, {Alexandra B.} and Huang, {Samuel J.} and Lebold, {Katie M.} and {De La Torre}, Ubaldo and Proskocil, {Becky J.} and Drake, {Matthew G.} and Hiroyuki Nakai and Fryer, {Allison D.} and Jacoby, {David B.}",
note = "Funding Information: This work was supported by the National Institutes of Health [HL131525, HL144008, AI152498, HL145906]. Funding Information: We thank the Oregon Health & Science University Advanced Light Microscopy core, especially Stephanie Kaech-Petrie, Brian Jenkins, and Crystal Shaw for their assistance with imaging. Many thanks to Dawen Cai for his guidance and technical support using nTracer software. The distribution of nTracer is partially supported by grant funding from NSF-1707316 Neunex MINT. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41598-022-08655-6",
language = "English (US)",
volume = "12",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}