@article{326d871eb2014b0e8789a98f007d1261,
title = "Mucus secretions from a conditionally reprogrammed primary endocervical cell culture",
abstract = "Objective: To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies. Design: Experimental. Setting: University research center. Animal(s): Female rhesus macaque (n = 2). Intervention(s): None. Main Outcome Measure(s): Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry. Result(s): We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix. Conclusion(s): Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.",
keywords = "Endocervix, mucus, primate, proteome",
author = "Daye Park and Ashok Reddy and Wilmarth, {Phillip A.} and Jeffrey Jensen and Leo Han",
note = "Funding Information: D.P. has nothing to disclose. A.P.R. has nothing to disclose. P.A.W. has nothing to disclose. J.T.J. has nothing to disclose. L.H. reports grants from Reproductive Scientist Development Program–NICHD grant K12 HD00849, The March of Dimes Foundation, American Society for Reproductive Medicine, American Board of Obstetrics and Gynecology, OHSU School of Medicine, Medical Foundation of Oregon and Oregon National Primate Research Center (core grant #P51OD011092), and NIH (grants P30EY010572, P30CA069533, and S10OD012246) for the submitted work. Funding Information: This research received support through the grant K12 HD000849, awarded to the Reproductive Scientist Development Program (RSDP) by the National Institute of Child Health and Human Development. In addition, this work received funding from the March of Dimes Foundation , American Society for Reproductive Medicine , the American Board of Obstetrics and Gynecology as part of the RSDP , and the Oregon Health & Science University School of Medicine , Medical Foundation of Oregon , and Oregon National Primate Research Center core grant number P51 OD011092. Mass spectrometry was done at the Oregon Health & Science University Proteomics Shared Resource with partial support from National Institute of Health grants P30EY010572, P30CA069533, and S10OD012246. Publisher Copyright: {\textcopyright} 2022 American Society for Reproductive Medicine",
year = "2022",
month = may,
doi = "10.1016/j.xfss.2022.03.003",
language = "English (US)",
volume = "3",
pages = "159--165",
journal = "F and S Science",
issn = "2666-335X",
publisher = "Elsevier BV",
number = "2",
}