Mucus secretions from a conditionally reprogrammed primary endocervical cell culture

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies. Design: Experimental. Setting: University research center. Animal(s): Female rhesus macaque (n = 2). Intervention(s): None. Main Outcome Measure(s): Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry. Result(s): We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix. Conclusion(s): Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalF and S Science
Volume3
Issue number2
DOIs
StatePublished - May 2022

Keywords

  • Endocervix
  • mucus
  • primate
  • proteome

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology

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