MRNA expression of ion channels in GnRH neurons

Subtype-specific regulation by 17β-estradiol

Martha A. Bosch, Karen J. Tonsfeldt, Oline Ronnekleiv

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Burst firing of neurons optimizes neurotransmitter release. GnRH neurons exhibit burst firing activity and T-type calcium channels, which are vital for burst firing activity, are regulated by 17β-estradiol (E2) in GnRH neurons. To further elucidate ion channel expression and E2 regulation during positive and negative feedback on GnRH neurosecretion, we used single cell RT-PCR and real-time qPCR to quantify channel mRNA expression in GnRH neurons. GFP-GnRH neurons expressed numerous ion channels important for burst firing activity. E2-treatment sufficient to induce an LH surge increased mRNA expression of HCN1 channels, which underlie the pacemaker current, the calcium-permeable CaV1.3, CaV2.2, CaV2.3 channels, and TRPC4 channels, which mediate the kisspeptin excitatory response. E2 also decreased mRNA expression of SK3 channels underlying the medium AHP current. Therefore, E2 exerts fundamental changes in ion channel expression in GnRH neurons, to prime them to respond to incoming stimuli with increased excitability at the time of the surge.

Original languageEnglish (US)
Pages (from-to)85-97
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume367
Issue number1-2
DOIs
StatePublished - Mar 10 2013

Fingerprint

Ion Channels
Gonadotropin-Releasing Hormone
Neurons
Estradiol
Messenger RNA
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
T-Type Calcium Channels
Kisspeptins
Neurosecretion
Feedback
Pacemakers
Neurotransmitter Agents
Real-Time Polymerase Chain Reaction
Calcium

Keywords

  • AHP
  • Channel subunits
  • E2
  • GnRH
  • GnRH neurons
  • GPR54
  • HCN
  • HVA
  • LH
  • LH surge
  • MRNA quantification
  • POA
  • QPCR
  • RT
  • Sc-PCR
  • SK
  • TRPC
  • ZT

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

MRNA expression of ion channels in GnRH neurons : Subtype-specific regulation by 17β-estradiol. / Bosch, Martha A.; Tonsfeldt, Karen J.; Ronnekleiv, Oline.

In: Molecular and Cellular Endocrinology, Vol. 367, No. 1-2, 10.03.2013, p. 85-97.

Research output: Contribution to journalArticle

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