MR imaging of inflammation during myelin-specific T cell-mediated autoimmune attack in the EAE mouse spinal cord

Kristine M. Robinson, Jeffrey M. Njus, Daniel A. Phillips, Thomas M. Proctor, William Rooney, Richard E. Jones

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Purpose: The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord. Procedures: T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in ∼100% iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument. Results: Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T2-weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells. Conclusions: These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells.

Original languageEnglish (US)
Pages (from-to)240-249
Number of pages10
JournalMolecular Imaging and Biology
Volume12
Issue number3
DOIs
Publication statusPublished - Jun 2010

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Keywords

  • EAE
  • Feridex
  • Ferumoxide
  • MRI
  • Multiple sclerosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

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