MR imaging of inflammation during myelin-specific T cell-mediated autoimmune attack in the EAE mouse spinal cord

Kristine M. Robinson, Jeffrey M. Njus, Daniel A. Phillips, Thomas M. Proctor, William Rooney, Richard E. Jones

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord. Procedures: T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in ∼100% iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument. Results: Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T2-weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells. Conclusions: These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells.

Original languageEnglish (US)
Pages (from-to)240-249
Number of pages10
JournalMolecular Imaging and Biology
Volume12
Issue number3
DOIs
StatePublished - Jun 2010

Fingerprint

Autoimmune Experimental Encephalomyelitis
Myelin Sheath
Spinal Cord
Magnetic Resonance Imaging
Inflammation
T-Lymphocytes
Iron
Magnetic Resonance Spectroscopy
Protamines
Multiple Sclerosis
Transfection
Animal Models
ferumoxides

Keywords

  • EAE
  • Feridex
  • Ferumoxide
  • MRI
  • Multiple sclerosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

MR imaging of inflammation during myelin-specific T cell-mediated autoimmune attack in the EAE mouse spinal cord. / Robinson, Kristine M.; Njus, Jeffrey M.; Phillips, Daniel A.; Proctor, Thomas M.; Rooney, William; Jones, Richard E.

In: Molecular Imaging and Biology, Vol. 12, No. 3, 06.2010, p. 240-249.

Research output: Contribution to journalArticle

Robinson, Kristine M. ; Njus, Jeffrey M. ; Phillips, Daniel A. ; Proctor, Thomas M. ; Rooney, William ; Jones, Richard E. / MR imaging of inflammation during myelin-specific T cell-mediated autoimmune attack in the EAE mouse spinal cord. In: Molecular Imaging and Biology. 2010 ; Vol. 12, No. 3. pp. 240-249.
@article{9ed91b4bf88b4a91b75b5d252af9cbb2,
title = "MR imaging of inflammation during myelin-specific T cell-mediated autoimmune attack in the EAE mouse spinal cord",
abstract = "Purpose: The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord. Procedures: T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in ∼100{\%} iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument. Results: Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T2-weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells. Conclusions: These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells.",
keywords = "EAE, Feridex, Ferumoxide, MRI, Multiple sclerosis",
author = "Robinson, {Kristine M.} and Njus, {Jeffrey M.} and Phillips, {Daniel A.} and Proctor, {Thomas M.} and William Rooney and Jones, {Richard E.}",
year = "2010",
month = "6",
doi = "10.1007/s11307-009-0272-6",
language = "English (US)",
volume = "12",
pages = "240--249",
journal = "Molecular Imaging and Biology",
issn = "1536-1632",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - MR imaging of inflammation during myelin-specific T cell-mediated autoimmune attack in the EAE mouse spinal cord

AU - Robinson, Kristine M.

AU - Njus, Jeffrey M.

AU - Phillips, Daniel A.

AU - Proctor, Thomas M.

AU - Rooney, William

AU - Jones, Richard E.

PY - 2010/6

Y1 - 2010/6

N2 - Purpose: The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord. Procedures: T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in ∼100% iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument. Results: Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T2-weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells. Conclusions: These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells.

AB - Purpose: The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord. Procedures: T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in ∼100% iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument. Results: Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T2-weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells. Conclusions: These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells.

KW - EAE

KW - Feridex

KW - Ferumoxide

KW - MRI

KW - Multiple sclerosis

UR - http://www.scopus.com/inward/record.url?scp=77956700485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956700485&partnerID=8YFLogxK

U2 - 10.1007/s11307-009-0272-6

DO - 10.1007/s11307-009-0272-6

M3 - Article

C2 - 19949987

AN - SCOPUS:77956700485

VL - 12

SP - 240

EP - 249

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

SN - 1536-1632

IS - 3

ER -