TY - JOUR
T1 - Mouse chromosome 7 harbors a quantitative trait locus for isoflurane minimum alveolar concentration
AU - Cascio, Michael
AU - Xing, Yilei
AU - Gong, Diane
AU - Popovich, John
AU - Eger, Edmond I.
AU - Sen, Saunak
AU - Peltz, Gary
AU - Sonner, James M.
PY - 2007/8
Y1 - 2007/8
N2 - BACKGROUND: The minimum alveolar concentration (MAC) of isoflurane is a quantitative trait because it varies continuously in a population. The location on the genome of genes or other genetic elements controlling quantiative traits is called quantitative trait loci (QTLs). In this study we sought to detect a quantitative trait locus underlying isoflurane MAC in mice. METHODS: To accomplish this, two inbred mouse strains differing in isoflurane MAC, the C57BL/6J and LP/J mouse strains, were bred through two generations to produce genetic recombination. These animals were genotyped for microsatellite markers. We also applied an independent, computational method for identifying QTL-regulating differences in isoflurane MAC. In this approach, the isoflurane MAC was measured in a panel of 19 inbred strains, and computationally searched for genomic intervals where the pattern of genetic variation, based on single nucleotide polymorphisms, correlated with the differences in isoflurane MAC among inbred strains. RESULTS AND CONCLUSIONS: Both methods of genetic analysis identified a QTL for isoflurane MAC that was located on the proximal part of mouse chromosome 7.
AB - BACKGROUND: The minimum alveolar concentration (MAC) of isoflurane is a quantitative trait because it varies continuously in a population. The location on the genome of genes or other genetic elements controlling quantiative traits is called quantitative trait loci (QTLs). In this study we sought to detect a quantitative trait locus underlying isoflurane MAC in mice. METHODS: To accomplish this, two inbred mouse strains differing in isoflurane MAC, the C57BL/6J and LP/J mouse strains, were bred through two generations to produce genetic recombination. These animals were genotyped for microsatellite markers. We also applied an independent, computational method for identifying QTL-regulating differences in isoflurane MAC. In this approach, the isoflurane MAC was measured in a panel of 19 inbred strains, and computationally searched for genomic intervals where the pattern of genetic variation, based on single nucleotide polymorphisms, correlated with the differences in isoflurane MAC among inbred strains. RESULTS AND CONCLUSIONS: Both methods of genetic analysis identified a QTL for isoflurane MAC that was located on the proximal part of mouse chromosome 7.
UR - http://www.scopus.com/inward/record.url?scp=34547569603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547569603&partnerID=8YFLogxK
U2 - 10.1213/01.ane.0000261277.65343.4e
DO - 10.1213/01.ane.0000261277.65343.4e
M3 - Article
C2 - 17646494
AN - SCOPUS:34547569603
SN - 0003-2999
VL - 105
SP - 381
EP - 385
JO - Anesthesia and analgesia
JF - Anesthesia and analgesia
IS - 2
ER -