Motifs that mediate dendritic targeting in hippocampal neurons: A comparison with basolateral targeting signals

M. A. Silverman, R. Peck, G. Glover, C. He, C. Carlin, Gary Banker

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

One model for dendritic protein sorting in neurons is based on parallels with basolateral targeting in Madin-Darby Canine Kidney (MDCK) epithelial cells. The goal of this study was to further evaluate this model by analyzing the neuronal targeting of several proteins that contain well-defined basolateral sorting motifs. When we expressed FcRγII-B2 and CD44, two basolateral markers whose sorting depends on dihydrophobic motifs, they were unpolarized in hippocampal neurons. We also assessed the localization of the Epidermal Growth Factor Receptor (EGFR), a basolateral protein whose sorting signal contains a proline-rich motif and two dihydrophobic motifs. EGFR was restricted to the dendrites in neurons and relied on the same sorting signal for proper targeting. These results show that the dendritic sorting machinery in neurons does not recognize dihydrophobic-based basolateral sorting signals. In contrast, the sorting signal present in EGFR directs both basolateral and dendritic targeting and defines a novel dendritic targeting motif.

Original languageEnglish (US)
Pages (from-to)173-180
Number of pages8
JournalMolecular and Cellular Neuroscience
Volume29
Issue number2
DOIs
Publication statusPublished - Jun 2005

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ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

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