TY - JOUR
T1 - Motifs that mediate dendritic targeting in hippocampal neurons
T2 - A comparison with basolateral targeting signals
AU - Silverman, M. A.
AU - Peck, R.
AU - Glover, G.
AU - He, C.
AU - Carlin, C.
AU - Banker, G.
N1 - Funding Information:
This research was supported by NIH grants NS17112 and MH66179 (to GB), NS48047 (to MS), and GM64243 and DK54178 (to CC). We thank B. Smoody for the preparation of neuronal cultures, J. Luisi Harp for assistance with fluorescence quantification, and M. Lasarev for assistance with the statistical analysis. S. Kaech, S. Das, and B. Sampo provided insightful comments on the manuscript.
PY - 2005/6
Y1 - 2005/6
N2 - One model for dendritic protein sorting in neurons is based on parallels with basolateral targeting in Madin-Darby Canine Kidney (MDCK) epithelial cells. The goal of this study was to further evaluate this model by analyzing the neuronal targeting of several proteins that contain well-defined basolateral sorting motifs. When we expressed FcRγII-B2 and CD44, two basolateral markers whose sorting depends on dihydrophobic motifs, they were unpolarized in hippocampal neurons. We also assessed the localization of the Epidermal Growth Factor Receptor (EGFR), a basolateral protein whose sorting signal contains a proline-rich motif and two dihydrophobic motifs. EGFR was restricted to the dendrites in neurons and relied on the same sorting signal for proper targeting. These results show that the dendritic sorting machinery in neurons does not recognize dihydrophobic-based basolateral sorting signals. In contrast, the sorting signal present in EGFR directs both basolateral and dendritic targeting and defines a novel dendritic targeting motif.
AB - One model for dendritic protein sorting in neurons is based on parallels with basolateral targeting in Madin-Darby Canine Kidney (MDCK) epithelial cells. The goal of this study was to further evaluate this model by analyzing the neuronal targeting of several proteins that contain well-defined basolateral sorting motifs. When we expressed FcRγII-B2 and CD44, two basolateral markers whose sorting depends on dihydrophobic motifs, they were unpolarized in hippocampal neurons. We also assessed the localization of the Epidermal Growth Factor Receptor (EGFR), a basolateral protein whose sorting signal contains a proline-rich motif and two dihydrophobic motifs. EGFR was restricted to the dendrites in neurons and relied on the same sorting signal for proper targeting. These results show that the dendritic sorting machinery in neurons does not recognize dihydrophobic-based basolateral sorting signals. In contrast, the sorting signal present in EGFR directs both basolateral and dendritic targeting and defines a novel dendritic targeting motif.
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U2 - 10.1016/j.mcn.2005.02.008
DO - 10.1016/j.mcn.2005.02.008
M3 - Article
C2 - 15911342
AN - SCOPUS:19444384837
SN - 1044-7431
VL - 29
SP - 173
EP - 180
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 2
ER -