TY - GEN
T1 - Morphometric subtyping for a panel of breast cancer cell lines
AU - Han, Ju
AU - Chang, Hang
AU - Fontenay, Gerald
AU - Wang, Nicholas J.
AU - Gray, Joe W.
AU - Parvin, Bahram
PY - 2009
Y1 - 2009
N2 - A panel of cell lines of diverse molecular background offers an improved model system for high-content screening, comparative analysis, and cell systems biology. A computational pipeline has been developed to collect images from cell-based assays, segment individual cells and colonies, represent segmented objects in a multidimensional space, and cluster them for identifying distinct subpopulations. While each segmentation strategy can vary for different imaging assays, representation and subpopulation analysis share a common thread. Application of this pipeline to a library of 41 breast cancer cell lines is demonstrated. These cell lines are grown in 2D and imaged through immunofluorescence microscopy. Subpopulations in this panel are identified and shown to correlate with previous subtyping literature that was derived from transcript data.
AB - A panel of cell lines of diverse molecular background offers an improved model system for high-content screening, comparative analysis, and cell systems biology. A computational pipeline has been developed to collect images from cell-based assays, segment individual cells and colonies, represent segmented objects in a multidimensional space, and cluster them for identifying distinct subpopulations. While each segmentation strategy can vary for different imaging assays, representation and subpopulation analysis share a common thread. Application of this pipeline to a library of 41 breast cancer cell lines is demonstrated. These cell lines are grown in 2D and imaged through immunofluorescence microscopy. Subpopulations in this panel are identified and shown to correlate with previous subtyping literature that was derived from transcript data.
UR - http://www.scopus.com/inward/record.url?scp=70449344422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70449344422&partnerID=8YFLogxK
U2 - 10.1109/ISBI.2009.5193168
DO - 10.1109/ISBI.2009.5193168
M3 - Conference contribution
AN - SCOPUS:70449344422
SN - 9781424439324
T3 - Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009
SP - 791
EP - 794
BT - Proceedings - 2009 IEEE International Symposium on Biomedical Imaging
T2 - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009
Y2 - 28 June 2009 through 1 July 2009
ER -