Morphometric subtyping for a panel of breast cancer cell lines

Ju Han, Hang Chang, Gerald Fontenay, Nicholas J. Wang, Joe W. Gray, Bahram Parvin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

A panel of cell lines of diverse molecular background offers an improved model system for high-content screening, comparative analysis, and cell systems biology. A computational pipeline has been developed to collect images from cell-based assays, segment individual cells and colonies, represent segmented objects in a multidimensional space, and cluster them for identifying distinct subpopulations. While each segmentation strategy can vary for different imaging assays, representation and subpopulation analysis share a common thread. Application of this pipeline to a library of 41 breast cancer cell lines is demonstrated. These cell lines are grown in 2D and imaged through immunofluorescence microscopy. Subpopulations in this panel are identified and shown to correlate with previous subtyping literature that was derived from transcript data.

Original languageEnglish (US)
Title of host publicationProceedings - 2009 IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2009
Pages791-794
Number of pages4
DOIs
StatePublished - 2009
Event2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009 - Boston, MA, United States
Duration: Jun 28 2009Jul 1 2009

Publication series

NameProceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009

Other

Other2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009
CountryUnited States
CityBoston, MA
Period6/28/097/1/09

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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    Han, J., Chang, H., Fontenay, G., Wang, N. J., Gray, J. W., & Parvin, B. (2009). Morphometric subtyping for a panel of breast cancer cell lines. In Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009 (pp. 791-794). [5193168] (Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009). https://doi.org/10.1109/ISBI.2009.5193168