Morphometric analysis of primordial follicle number in pigtailed monkey ovaries: Symmetry and relationship with age

Paul B. Miller, Jay S. Charleston, David Battaglia, Nancy A. Klein, Michael R. Soules

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We previously described a modern, three-dimensional counting method for determining primordial follicle (PF) numbers in primate ovaries using a combination of fractionator and physical dissector techniques. The purposes of our current study were 1) to apply our method to describe intraindividual differences in PF numbers between ovaries and 2) perform a linear regression analysis of age versus mean PF number per ovary. Ovaries from 16 pigtailed monkeys (Macaca nemestrina) age 0.85-12.5 yr were examined. Both ovaries were available from 11 subjects. The difference between ovaries ranged from 2% to 22% (mean ± SD, 10 ± 7%) and was not statistically significant. Regression analysis of data from all 16 subjects displayed a log-linear relationship according to the equation log N(a) = 4.8542 0.0714(age) where N(a) is the number of PF at a given chronological age. The fit for this model was highly significant (r2 = 0.73, p ≤ 0.0001). Extrapolation of the model suggests that there are 71 483 PF in each ovary at the time of birth. We conclude that right and left ovaries differ little and that PF numbers follow a log-linear rate of decline during the reproductive years in this species.

Original languageEnglish (US)
Pages (from-to)553-556
Number of pages4
JournalBiology of Reproduction
Volume61
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

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Macaca nemestrina
Ovary
Regression Analysis
Primates
Linear Models
Parturition

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Morphometric analysis of primordial follicle number in pigtailed monkey ovaries : Symmetry and relationship with age. / Miller, Paul B.; Charleston, Jay S.; Battaglia, David; Klein, Nancy A.; Soules, Michael R.

In: Biology of Reproduction, Vol. 61, No. 2, 1999, p. 553-556.

Research output: Contribution to journalArticle

Miller, Paul B. ; Charleston, Jay S. ; Battaglia, David ; Klein, Nancy A. ; Soules, Michael R. / Morphometric analysis of primordial follicle number in pigtailed monkey ovaries : Symmetry and relationship with age. In: Biology of Reproduction. 1999 ; Vol. 61, No. 2. pp. 553-556.
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