Morphologic evidence that neurokinin B modulates gonadotropin-releasing hormone secretion via neurokinin 3 receptors in the rat median eminence

Sally J. Krajewski, Miranda J. Anderson, Lulu Iles-Shih, Kyung J. Chen, Henryk Urbanski, Naomi E. Rance

    Research output: Contribution to journalArticle

    135 Citations (Scopus)

    Abstract

    Recent studies suggest that arcuate neurokinin B (NKB) neurons play a role in the regulation of gonadotropin secretion, but there is little information on the relationship between these neurons and the hypothalamic reproductive axis. In the present study, dual-label fluorescent immunohistochemistry was used to visualize the relationship between gonadotropin-releasing hormone (GnRH) neurons and either proNKB or NK3 receptor (NK3R) immunoreactivity. Immunocytochemistry was also combined with i.p. injections of the fluorescent retrograde tracer aminostilbamidine to determine whether arcuate neuroendocrine neurons expressed either proNKB or NK3R. A dense interweaving and close apposition of GnRH and proNKB-immunoreactive (ir) fibers was observed within the rat median eminence, where GnRH axons expressed NK 3R immunoreactivity. These data provide morphological evidence that NKB neurons could influence GnRH secretion via interaction with NK3R in the rat median eminence. Colocalization of GnRH and NK3R was also identified in fiber tracts converging within the organum vasculosum of the lamina terminalis. In contrast, only a small number (16%) of GnRH-ir somata exhibited NK3R staining. ProNKB and NK3R-ir somata were identified within the arcuate nucleus, but none of these neurons were labeled by aminostilbamidine. Thus, we found no evidence that arcuate NKB neurons project to the primary capillary plexus of the portal system. Arcuate neuroendocrine neurons, however, were surrounded and closely apposed by proNKB-ir puncta and fibers. These data suggest that NKB neurons could indirectly influence anterior pituitary function by inputs to arcuate neuroendocrine neurons, but through a receptor other than NK3R. Our results provide an anatomic framework for putative interactions between NKB neurons and the hypothalamic reproductive axis.

    Original languageEnglish (US)
    Pages (from-to)372-386
    Number of pages15
    JournalJournal of Comparative Neurology
    Volume489
    Issue number3
    DOIs
    StatePublished - Aug 29 2005

    Fingerprint

    Neurokinin-3 Receptors
    Neurokinin B
    Median Eminence
    Gonadotropin-Releasing Hormone
    Neurons
    Carisoprodol
    Immunohistochemistry
    LHRH Receptors
    Portal System
    Arcuate Nucleus of Hypothalamus
    Gonadotropins
    Axons

    Keywords

    • Arcuate nucleus
    • Estrogen
    • Hypothalamus
    • Luteinizing hormone
    • Neuroendocrine
    • Tachykinin

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Morphologic evidence that neurokinin B modulates gonadotropin-releasing hormone secretion via neurokinin 3 receptors in the rat median eminence. / Krajewski, Sally J.; Anderson, Miranda J.; Iles-Shih, Lulu; Chen, Kyung J.; Urbanski, Henryk; Rance, Naomi E.

    In: Journal of Comparative Neurology, Vol. 489, No. 3, 29.08.2005, p. 372-386.

    Research output: Contribution to journalArticle

    Krajewski, Sally J. ; Anderson, Miranda J. ; Iles-Shih, Lulu ; Chen, Kyung J. ; Urbanski, Henryk ; Rance, Naomi E. / Morphologic evidence that neurokinin B modulates gonadotropin-releasing hormone secretion via neurokinin 3 receptors in the rat median eminence. In: Journal of Comparative Neurology. 2005 ; Vol. 489, No. 3. pp. 372-386.
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    abstract = "Recent studies suggest that arcuate neurokinin B (NKB) neurons play a role in the regulation of gonadotropin secretion, but there is little information on the relationship between these neurons and the hypothalamic reproductive axis. In the present study, dual-label fluorescent immunohistochemistry was used to visualize the relationship between gonadotropin-releasing hormone (GnRH) neurons and either proNKB or NK3 receptor (NK3R) immunoreactivity. Immunocytochemistry was also combined with i.p. injections of the fluorescent retrograde tracer aminostilbamidine to determine whether arcuate neuroendocrine neurons expressed either proNKB or NK3R. A dense interweaving and close apposition of GnRH and proNKB-immunoreactive (ir) fibers was observed within the rat median eminence, where GnRH axons expressed NK 3R immunoreactivity. These data provide morphological evidence that NKB neurons could influence GnRH secretion via interaction with NK3R in the rat median eminence. Colocalization of GnRH and NK3R was also identified in fiber tracts converging within the organum vasculosum of the lamina terminalis. In contrast, only a small number (16{\%}) of GnRH-ir somata exhibited NK3R staining. ProNKB and NK3R-ir somata were identified within the arcuate nucleus, but none of these neurons were labeled by aminostilbamidine. Thus, we found no evidence that arcuate NKB neurons project to the primary capillary plexus of the portal system. Arcuate neuroendocrine neurons, however, were surrounded and closely apposed by proNKB-ir puncta and fibers. These data suggest that NKB neurons could indirectly influence anterior pituitary function by inputs to arcuate neuroendocrine neurons, but through a receptor other than NK3R. Our results provide an anatomic framework for putative interactions between NKB neurons and the hypothalamic reproductive axis.",
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    AU - Iles-Shih, Lulu

    AU - Chen, Kyung J.

    AU - Urbanski, Henryk

    AU - Rance, Naomi E.

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