TY - JOUR
T1 - Morphologic and immunohistochemical characterization of granulomas in the nucleotide oligomerization domain 2-related disorders Blau syndrome and Crohn disease
AU - Janssen, Carl E.I.
AU - Rose, Carlos D.
AU - De Hertogh, Gert
AU - Martin, Tammy M.
AU - Bader Meunier, Brigitte
AU - Cimaz, Rolando
AU - Harjacek, Miroslav
AU - Quartier, Pierre
AU - Ten Cate, Rebecca
AU - Thomee, Caroline
AU - Desmet, Valeer J.
AU - Fischer, Alain
AU - Roskams, Tania
AU - Wouters, Carine H.
N1 - Funding Information:
C.H.W. was awarded an unrestricted grant from GlaxoSmithKline to study Blau syndrome, and C.D.R. is a coinvestigator on that grant. T.M. is supported by the National Institutes of Health and the Research to Prevent Blindness.
PY - 2012/4
Y1 - 2012/4
N2 - Background: Blau syndrome (BS) and Crohn disease (CD) are both characterized by granulomatous inflammation and related to nucleotide oligomerization domain 2 (NOD2) mutations. Objective: This study aimed to define the morphologic and immunohistochemical characteristics of granulomas in patients with NOD2-related BS and CD. Methods: Granuloma-containing biopsy specimens from 6 patients with BS and 7 pediatric patients with CD carrying NOD2 mutations or single nucleotide polymorphisms were studied for morphology, cellular composition, and cytokine expression by using hematoxylin and eosin staining and immunohistochemistry. Results: Biopsy specimens from patients with BS typically showed polycyclic granulomas with large lymphocytic coronas, extensive emperipolesis of lymphocytes within multinucleated giant cells (MGCs), MGC death, and fibrinoid necrosis and fibrosis. In contrast, biopsy specimens from patients with CD showed simple granulomas with subtle/absent lymphocytic coronas, sclerosis of the surrounding tissue, and polymorphonuclear cells. Findings found to be similar in all granulomas were as follows: CD68 and HLA-DR expression by epithelioid cells, monocyte-macrophage lineage cells and MGCs, increased lymphocytic HLA-DR expression, increased CD4 +/CD8 + T-cell ratio, and CD20 + B lymphocytes evenly distributed within and around granulomas. In both patient groups prominent IFN-γ expression was found in and around granulomas, and TNF-α and IL-23 receptor expression was moderate. IL-6, IL-17, and TGF-β expression was prominent in granulomas from patients with BS but sporadic in granulomas from patients with CD. IL-10 expression was absent. Conclusion: Granulomas from patients with BS and granulomas from patients with NOD2-associated CD show distinct morphologic features and cytokine expression patterns, suggesting that the T H17 axis might be involved in the pathogenesis of BS, whereas T H1 is important in both patients with BS and patients with CD.
AB - Background: Blau syndrome (BS) and Crohn disease (CD) are both characterized by granulomatous inflammation and related to nucleotide oligomerization domain 2 (NOD2) mutations. Objective: This study aimed to define the morphologic and immunohistochemical characteristics of granulomas in patients with NOD2-related BS and CD. Methods: Granuloma-containing biopsy specimens from 6 patients with BS and 7 pediatric patients with CD carrying NOD2 mutations or single nucleotide polymorphisms were studied for morphology, cellular composition, and cytokine expression by using hematoxylin and eosin staining and immunohistochemistry. Results: Biopsy specimens from patients with BS typically showed polycyclic granulomas with large lymphocytic coronas, extensive emperipolesis of lymphocytes within multinucleated giant cells (MGCs), MGC death, and fibrinoid necrosis and fibrosis. In contrast, biopsy specimens from patients with CD showed simple granulomas with subtle/absent lymphocytic coronas, sclerosis of the surrounding tissue, and polymorphonuclear cells. Findings found to be similar in all granulomas were as follows: CD68 and HLA-DR expression by epithelioid cells, monocyte-macrophage lineage cells and MGCs, increased lymphocytic HLA-DR expression, increased CD4 +/CD8 + T-cell ratio, and CD20 + B lymphocytes evenly distributed within and around granulomas. In both patient groups prominent IFN-γ expression was found in and around granulomas, and TNF-α and IL-23 receptor expression was moderate. IL-6, IL-17, and TGF-β expression was prominent in granulomas from patients with BS but sporadic in granulomas from patients with CD. IL-10 expression was absent. Conclusion: Granulomas from patients with BS and granulomas from patients with NOD2-associated CD show distinct morphologic features and cytokine expression patterns, suggesting that the T H17 axis might be involved in the pathogenesis of BS, whereas T H1 is important in both patients with BS and patients with CD.
KW - Blau syndrome
KW - Crohn disease
KW - Nucleotide oligomerization domain 2
KW - T 17 cell
KW - granuloma
KW - monocyte macrophage lineage
KW - multinucleated giant cell
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U2 - 10.1016/j.jaci.2012.02.004
DO - 10.1016/j.jaci.2012.02.004
M3 - Article
C2 - 22464675
AN - SCOPUS:84859145549
SN - 0091-6749
VL - 129
SP - 1076
EP - 1084
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
IS - 4
ER -