TY - JOUR
T1 - Morphine Inhibits the Pituitary-Adrenal Response to Ovine Corticotropin-Releasing Hormone in Normal Subjects
AU - Rittmaster, Roger S.
AU - Cutler, Gordon B.
AU - Sobel, Douglas O.
AU - Goldstein, David S.
AU - Koppelman, Michele C.S.
AU - Loriaux, D. Lynn
AU - Chrousos, George P.
PY - 1985/5
Y1 - 1985/5
N2 - To determine the locus of opiate modulation of ACTH secretion, 11 normal subjects were given ovine cortico-tropin-releasing hormone (CRH) 30 min after receiving either placebo or morphine sulfate. Plasma ACTH, cortisol, arginine vasopressin (AVP), epinephrine, norepinephrine, and CRH were measured 30 min before and up to 150 min after CRH administration. Morphine blunted the ACTH response for the first 60 min and cortisol response for the first 90 min after CRH administration. Morphine did not lower arginine vasopressin or cate-cholamine levels. To determine whether morphine’s effect on ACTH and cortisol was due to a direct action on the corticotrophcell, dispersed rat pituitary cells were perifused with medium containing 1 μg/ml morphine sulfate or medium alone. Morphine had no effect on the ACTH response of these cells to 10 nM CRH pulses. Similarly, morphine had no effect on ACTH production by dispersed rat pituitary cells in monolayer culture in response to 90- and 180-min incubations with 5 nM CRH. We conclude that morphine blunts the early response of the pituitary gland to CRH in vivo Based on the lack of a direct effect of morphine on rat pituitary cells in vitro, we postulate that morphine given in vivo may modulate the pituitary ACTH response to CRH through other suprapituitary factors.
AB - To determine the locus of opiate modulation of ACTH secretion, 11 normal subjects were given ovine cortico-tropin-releasing hormone (CRH) 30 min after receiving either placebo or morphine sulfate. Plasma ACTH, cortisol, arginine vasopressin (AVP), epinephrine, norepinephrine, and CRH were measured 30 min before and up to 150 min after CRH administration. Morphine blunted the ACTH response for the first 60 min and cortisol response for the first 90 min after CRH administration. Morphine did not lower arginine vasopressin or cate-cholamine levels. To determine whether morphine’s effect on ACTH and cortisol was due to a direct action on the corticotrophcell, dispersed rat pituitary cells were perifused with medium containing 1 μg/ml morphine sulfate or medium alone. Morphine had no effect on the ACTH response of these cells to 10 nM CRH pulses. Similarly, morphine had no effect on ACTH production by dispersed rat pituitary cells in monolayer culture in response to 90- and 180-min incubations with 5 nM CRH. We conclude that morphine blunts the early response of the pituitary gland to CRH in vivo Based on the lack of a direct effect of morphine on rat pituitary cells in vitro, we postulate that morphine given in vivo may modulate the pituitary ACTH response to CRH through other suprapituitary factors.
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U2 - 10.1210/jcem-60-5-891
DO - 10.1210/jcem-60-5-891
M3 - Article
C2 - 2984235
AN - SCOPUS:0021813977
SN - 0021-972X
VL - 60
SP - 891
EP - 895
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 5
ER -