Monocyte chemotactic activity induced by intravitreal endotoxin

Research output: Contribution to journalArticle

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Abstract

In order to clarify the factors responsible for the cellular infiltrate characteristic of anterior uveitis, the authors have induced inflammation in rabbits by the intravitreal injection of 100 ng of Escherichia coli or Salmonella endotoxin (ET). A 2% concentration of aqueous humor 18 to 24 hr after ET consistently induced monocyte migration as measured in modified Boyden chambers. Activity was significantly greater in these samples than in aqueous after saline injection or 3 hr after endotoxin injection (prior to cellular infiltrate). Using either sephadex G-75 molecular sieve chromatography or a cibacron blue column, the vast majority of migratory activity co-eluted with albumin. Serum albumin, however, at a comparable concentration did not induce migration. Activity was largely heat- and acid-stable and was maximal in the presence of a concentration gradient, indicating that it was chemotactic rather than chemokinetic. A second peak of activity eluted from the G-75 column just prior to a marker with molecular weight of 427 and was also present in eluates from normal aqueous humor. Chloroform:methanol extraction, radioimmunoassay, and high performance liquid chromatography indicated that a small portion of the chemotactic activity could be ascribed to lipid including leukotriene B4. In contrast to the prominence of complement (C5a) derived chemotactic activity resulting from intravenous ET, C5a was not a major contributor to aqueous chemotactic activity subsequent to local ET. These observations demonstrate that leukocyte migration factors in aqueous humor can be characterized and compared. This approach can be used to test the hypothesis that subsets of anterior uveal inflammation might be distinguished on the basis of associated chemotactic factors.

Original languageEnglish (US)
Pages (from-to)1267-1273
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume26
Issue number9
StatePublished - 1985
Externally publishedYes

Fingerprint

Endotoxins
Aqueous Humor
Monocytes
Complement C5a
Inflammation
Anterior Uveitis
Intravitreal Injections
Injections
Leukotriene B4
Chemotactic Factors
Chloroform
Serum Albumin
Radioimmunoassay
Gel Chromatography
Methanol
Albumins
Leukocytes
Hot Temperature
Molecular Weight
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Monocyte chemotactic activity induced by intravitreal endotoxin. / Rosenbaum, James (Jim); Raymond, W.

In: Investigative Ophthalmology and Visual Science, Vol. 26, No. 9, 1985, p. 1267-1273.

Research output: Contribution to journalArticle

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