Monoclonal antibody DS6 detects a tumor-associated sialoglycotope expressed on human serous ovarian carcinomas

Kelly P. Kearse, Nancy L. Smith, Diane A. Semer, Lynsey Eagles, James L. Finley, Steven (Steve) Kazmierczak, Charles J. Kovacs, Arthur A. Rodriguez, Anne E. Kellogg-Wennerberg

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A newly developed murine monoclonal antibody, DS6, immunohistochemically reacts with an antigen, CA6, that is expressed by human serous ovarian carcinomas but not by normal ovarian surface epithelium or mesothelium. CA6 has a limited distribution in normal adult tissues and is most characteristically detected in fallopian tube epithelium, inner urothelium and type 2 pneumocytes. Pre-treatment of tissue sections with either periodic acid or neuraminidase from Vibrio cholerae abolishes immunoreactivity with DS6, indicating that CA6 is a neuraminidase-sensitive and periodic acid-sensitive sialic acid glycoconjugate ('sialoglycotope'). SDS-PAGE of OVCAR5 cell lysates has revealed that the CA6 epitope is expressed on an 80 kDa non-disulfide-linked glycoprotein containing N-linked oligosaccharides. Two-dimensional non-equilibrium pH gradient gel electrophoresis indicates an isoelectric point of approximately 6.2 to 6.5. Comparison of the immunohistochemical distribution of CA6 in human serous ovarian adenocarcinomas has revealed similarities to that of CAI25; however, distinct differences and some complementarity of antigen expression were revealed by double-label, 2-color immunohistochemical studies. The DS6-detected CA6 antigen appears to be distinct from other well-characterized tumor-associated antigens, including MUC1, CA125 and the histo-blood group-related antigens sLea, sLex and sTn. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)866-872
Number of pages7
JournalInternational Journal of Cancer
Volume88
Issue number6
DOIs
StatePublished - Dec 15 2000
Externally publishedYes

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Periodic Acid
Epithelium
Monoclonal Antibodies
Neuraminidase
Carcinoma
Antigens
Alveolar Epithelial Cells
Urothelium
Neoplasms
Proton-Motive Force
Glycoconjugates
Vibrio cholerae
Fallopian Tubes
Normal Distribution
Isoelectric Point
N-Acetylneuraminic Acid
Neoplasm Antigens
Blood Group Antigens
Oligosaccharides
Electrophoresis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Monoclonal antibody DS6 detects a tumor-associated sialoglycotope expressed on human serous ovarian carcinomas. / Kearse, Kelly P.; Smith, Nancy L.; Semer, Diane A.; Eagles, Lynsey; Finley, James L.; Kazmierczak, Steven (Steve); Kovacs, Charles J.; Rodriguez, Arthur A.; Kellogg-Wennerberg, Anne E.

In: International Journal of Cancer, Vol. 88, No. 6, 15.12.2000, p. 866-872.

Research output: Contribution to journalArticle

Kearse, Kelly P. ; Smith, Nancy L. ; Semer, Diane A. ; Eagles, Lynsey ; Finley, James L. ; Kazmierczak, Steven (Steve) ; Kovacs, Charles J. ; Rodriguez, Arthur A. ; Kellogg-Wennerberg, Anne E. / Monoclonal antibody DS6 detects a tumor-associated sialoglycotope expressed on human serous ovarian carcinomas. In: International Journal of Cancer. 2000 ; Vol. 88, No. 6. pp. 866-872.
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T1 - Monoclonal antibody DS6 detects a tumor-associated sialoglycotope expressed on human serous ovarian carcinomas

AU - Kearse, Kelly P.

AU - Smith, Nancy L.

AU - Semer, Diane A.

AU - Eagles, Lynsey

AU - Finley, James L.

AU - Kazmierczak, Steven (Steve)

AU - Kovacs, Charles J.

AU - Rodriguez, Arthur A.

AU - Kellogg-Wennerberg, Anne E.

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N2 - A newly developed murine monoclonal antibody, DS6, immunohistochemically reacts with an antigen, CA6, that is expressed by human serous ovarian carcinomas but not by normal ovarian surface epithelium or mesothelium. CA6 has a limited distribution in normal adult tissues and is most characteristically detected in fallopian tube epithelium, inner urothelium and type 2 pneumocytes. Pre-treatment of tissue sections with either periodic acid or neuraminidase from Vibrio cholerae abolishes immunoreactivity with DS6, indicating that CA6 is a neuraminidase-sensitive and periodic acid-sensitive sialic acid glycoconjugate ('sialoglycotope'). SDS-PAGE of OVCAR5 cell lysates has revealed that the CA6 epitope is expressed on an 80 kDa non-disulfide-linked glycoprotein containing N-linked oligosaccharides. Two-dimensional non-equilibrium pH gradient gel electrophoresis indicates an isoelectric point of approximately 6.2 to 6.5. Comparison of the immunohistochemical distribution of CA6 in human serous ovarian adenocarcinomas has revealed similarities to that of CAI25; however, distinct differences and some complementarity of antigen expression were revealed by double-label, 2-color immunohistochemical studies. The DS6-detected CA6 antigen appears to be distinct from other well-characterized tumor-associated antigens, including MUC1, CA125 and the histo-blood group-related antigens sLea, sLex and sTn. (C) 2000 Wiley-Liss, Inc.

AB - A newly developed murine monoclonal antibody, DS6, immunohistochemically reacts with an antigen, CA6, that is expressed by human serous ovarian carcinomas but not by normal ovarian surface epithelium or mesothelium. CA6 has a limited distribution in normal adult tissues and is most characteristically detected in fallopian tube epithelium, inner urothelium and type 2 pneumocytes. Pre-treatment of tissue sections with either periodic acid or neuraminidase from Vibrio cholerae abolishes immunoreactivity with DS6, indicating that CA6 is a neuraminidase-sensitive and periodic acid-sensitive sialic acid glycoconjugate ('sialoglycotope'). SDS-PAGE of OVCAR5 cell lysates has revealed that the CA6 epitope is expressed on an 80 kDa non-disulfide-linked glycoprotein containing N-linked oligosaccharides. Two-dimensional non-equilibrium pH gradient gel electrophoresis indicates an isoelectric point of approximately 6.2 to 6.5. Comparison of the immunohistochemical distribution of CA6 in human serous ovarian adenocarcinomas has revealed similarities to that of CAI25; however, distinct differences and some complementarity of antigen expression were revealed by double-label, 2-color immunohistochemical studies. The DS6-detected CA6 antigen appears to be distinct from other well-characterized tumor-associated antigens, including MUC1, CA125 and the histo-blood group-related antigens sLea, sLex and sTn. (C) 2000 Wiley-Liss, Inc.

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