Monoclonal antibodies with high affinity for spiroperidol

R. R. Luedtke, M. Korner, Kim Neve, P. B. Molinoff

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A diverse panel of monoclonal antibodies was obtained from BALB/c mice immunized with two haptens structurally related to spiroperidol (SPD). Bromoacetyl derivatives of aminospiroperidol (NH2SPD) and N-amino-phenethylspiroperidol (NAPS) were synthesized to couple the haptens covalently to a protein carrier for immunization, thereby maintaining the butyrophenone portion of the immunogen. Hybridomas were selected based on their ability to secrete antibody that binds [3H]SPD with high affinity. Equilibrium dissociation constants for these antibodies ranged from 0.2 to > 100 nM. The antigen binding sites of the anti-NH2SPD and anti-NAPS antibodies were characterized in studies of the inhibition of the binding of [3H]-SPD by a series of ligands that are either (a) structurally related to SPD or (b) structurally unrelated to the butyrophenones but known to be selective antagonists of the D2 subtype of dopamine receptor. Based on the patterns of inhibition of the binding of [3H]SPD by these compounds, 12 classes of antibody combining sites were identified. Most of these antibodies bound butyrophenones with high affinity. One-anti-NH2SPD and four anti-NAPS antibodies also bound domperidone, a nonbutyrophenone that has a high affinity for D2 receptors. None of the antibodies bound clebopride or sulpiride, D2-selective antagonists of the benzamide class, or the agonist dopamine.

Original languageEnglish (US)
Pages (from-to)1253-1262
Number of pages10
JournalJournal of Neurochemistry
Volume50
Issue number4
StatePublished - 1988
Externally publishedYes

Fingerprint

Spiperone
Butyrophenones
Monoclonal Antibodies
Antibodies
Haptens
Binding Sites
Immunologic Techniques
Domperidone
Sulpiride
Immunoglobulin Isotypes
Immunization
Dopamine Agonists
Hybridomas
Dopamine Receptors
Carrier Proteins
Ligands
Antigens
Derivatives

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Luedtke, R. R., Korner, M., Neve, K., & Molinoff, P. B. (1988). Monoclonal antibodies with high affinity for spiroperidol. Journal of Neurochemistry, 50(4), 1253-1262.

Monoclonal antibodies with high affinity for spiroperidol. / Luedtke, R. R.; Korner, M.; Neve, Kim; Molinoff, P. B.

In: Journal of Neurochemistry, Vol. 50, No. 4, 1988, p. 1253-1262.

Research output: Contribution to journalArticle

Luedtke, RR, Korner, M, Neve, K & Molinoff, PB 1988, 'Monoclonal antibodies with high affinity for spiroperidol', Journal of Neurochemistry, vol. 50, no. 4, pp. 1253-1262.
Luedtke RR, Korner M, Neve K, Molinoff PB. Monoclonal antibodies with high affinity for spiroperidol. Journal of Neurochemistry. 1988;50(4):1253-1262.
Luedtke, R. R. ; Korner, M. ; Neve, Kim ; Molinoff, P. B. / Monoclonal antibodies with high affinity for spiroperidol. In: Journal of Neurochemistry. 1988 ; Vol. 50, No. 4. pp. 1253-1262.
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