Monoclonal antibodies to luteinizing hormone-releasing hormone: Production, characterization, and immunocytochemical application

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    Abstract

    Luteinizing hormone-releasing hormone (LHRH) was conjugated to bovine thyroglobulin and used to immunize a BALB/c mouse. Spleen lymphocytes were subsequently fused to SP2/0 myeloma cells and two of the resulting hybridoma clones were found to produce high titer antibodies to LHRH (HU4H and HU11B); both belonged to the IgG1 subclass. Characterization of the monoclonal antibodies revealed that HU4H and HU11B have conformational and sequential specificity to LHRH, respectively, and that neither one shows significant immunoactivity with pro-LHRH. The value of these antibodies in immunocytochemical applications is demonstrated by their ability to cause intense specific staining of LHRH neuronal cell bodies and fibers in brain sections from several mammalian species.

    Original languageEnglish (US)
    Pages (from-to)681-686
    Number of pages6
    JournalBiology of reproduction
    Volume44
    Issue number4
    DOIs
    StatePublished - Jan 1 1991

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    ASJC Scopus subject areas

    • Reproductive Medicine
    • Cell Biology

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