Monitoring Hypoxia Induced Changes in Cochlear Blood Flow and Hemoglobin Concentration Using a Combined Dual-Wavelength Laser Speckle Contrast Imaging and Doppler Optical Microangiography System

Roberto Reif, Jia Qin, Lei Shi, Suzan Dziennis, Zhongwei Zhi, Alfred Nuttall, Ruikang K. Wang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A synchronized dual-wavelength laser speckle contrast imaging (DWLSCI) system and a Doppler optical microangiography (DOMAG) system was developed to determine several ischemic parameters in the cochlea due to a systemic hypoxic challenge. DWLSCI can obtain two-dimensional data, and was used to determine the relative changes in cochlear blood flow, and change in the concentrations of oxyhemoglobin (HbO), deoxyhemoglobin (Hb) and total hemoglobin (HbT) in mice. DOMAG can obtain three-dimensional data, and was used to determine the changes in cochlear blood flow with single vessel resolution. It was demonstrated that during a hypoxic challenge there was an increase in the concentrations of Hb, a decrease in the concentrations of HbO and cochlear blood flow, and a slight decrease in the concentration of HbT. Also, the rate of change in the concentrations of Hb and HbO was quantified during and after the hypoxic challenge. The ability to simultaneously measure these ischemic parameters with high spatio-temporal resolution will allow the detailed quantitative analysis of several hearing disorders, and will be useful for diagnosing and developing treatments.

Original languageEnglish (US)
Article numbere52041
JournalPLoS One
Volume7
Issue number12
DOIs
StatePublished - Dec 18 2012

Fingerprint

Optical Devices
Cochlea
Speckle
Optical systems
blood flow
wavelengths
hypoxia
hemoglobin
Hemoglobins
Lasers
Blood
image analysis
Imaging techniques
Wavelength
lasers
hearing disorders
Monitoring
monitoring
Oxyhemoglobins
Audition

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Monitoring Hypoxia Induced Changes in Cochlear Blood Flow and Hemoglobin Concentration Using a Combined Dual-Wavelength Laser Speckle Contrast Imaging and Doppler Optical Microangiography System. / Reif, Roberto; Qin, Jia; Shi, Lei; Dziennis, Suzan; Zhi, Zhongwei; Nuttall, Alfred; Wang, Ruikang K.

In: PLoS One, Vol. 7, No. 12, e52041, 18.12.2012.

Research output: Contribution to journalArticle

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