Monitoring human melanocytic cell responses to piperine using multispectral imaging

Ravikant Samatham, Kevin G. Phillips, Julia Sonka, Aznegashe Yelma, Neha Reddy, Meenakshi Vanka, Philippe Thuillier, Amala Soumyanath, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Vitiligo is a depigmentary disease characterized by melanocyte loss attributed most commonly to autoimmune mechanisms. Currently vitiligo has a high incidence (1% worldwide) but a poor set of treatment options. Piperine, a compound found in black pepper, is a potential treatment for the depigmentary skin disease vitiligo, due to its ability to stimulate mouse epidermal melanocyte proliferation in vitro and in vivo. The present study investigates the use of multispectral imaging and an image processing technique based on local contrast to quantify the stimulatory effects of piperine on human melanocyte proliferation in reconstructed epidermis. We demonstrate the ability of the imaging method to quantify increased pigmentation in response to piperine treatment. The quantization of melanocyte stimulation by the proposed imaging technique illustrates the potential use of this technology to quickly assess therapeutic responses of vitiligo tissue culture models to treatment non-invasively.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7883
DOIs
StatePublished - 2011
EventPhotonic Therapeutics and Diagnostics VII - San Francisco, CA, United States
Duration: Jan 22 2011Jan 24 2011

Other

OtherPhotonic Therapeutics and Diagnostics VII
CountryUnited States
CitySan Francisco, CA
Period1/22/111/24/11

Fingerprint

piperine
Vitiligo
Melanocytes
Imaging techniques
Monitoring
cells
Tissue culture
Piper nigrum
peppers
epidermis
Skin
Pigmentation
Image processing
stimulation
Skin Diseases
Epidermis
imaging techniques
mice
image processing
incidence

Keywords

  • image processing
  • melanin
  • multispectral imaging
  • tissue culture
  • vitiligo

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Samatham, R., Phillips, K. G., Sonka, J., Yelma, A., Reddy, N., Vanka, M., ... Jacques, S. (2011). Monitoring human melanocytic cell responses to piperine using multispectral imaging. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7883). [788309] https://doi.org/10.1117/12.875620

Monitoring human melanocytic cell responses to piperine using multispectral imaging. / Samatham, Ravikant; Phillips, Kevin G.; Sonka, Julia; Yelma, Aznegashe; Reddy, Neha; Vanka, Meenakshi; Thuillier, Philippe; Soumyanath, Amala; Jacques, Steven.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7883 2011. 788309.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Samatham, R, Phillips, KG, Sonka, J, Yelma, A, Reddy, N, Vanka, M, Thuillier, P, Soumyanath, A & Jacques, S 2011, Monitoring human melanocytic cell responses to piperine using multispectral imaging. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7883, 788309, Photonic Therapeutics and Diagnostics VII, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.875620
Samatham R, Phillips KG, Sonka J, Yelma A, Reddy N, Vanka M et al. Monitoring human melanocytic cell responses to piperine using multispectral imaging. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7883. 2011. 788309 https://doi.org/10.1117/12.875620
Samatham, Ravikant ; Phillips, Kevin G. ; Sonka, Julia ; Yelma, Aznegashe ; Reddy, Neha ; Vanka, Meenakshi ; Thuillier, Philippe ; Soumyanath, Amala ; Jacques, Steven. / Monitoring human melanocytic cell responses to piperine using multispectral imaging. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7883 2011.
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