Molecular structure of the uvrC gene of Escherichia coli

Identification of DNA sequences required for transcription of the uvrC gene

Surendra Sharma, William Dowhan, Robb Moses

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have carried out experiments to identify the regulatory regions of the uvrC gene of Escherichia coli. A uvrC+ plasmid, pUV7, containing the intact transcriptional unit for the uvrC gene, was used to subclone either the structural gene or combinations of the structural gene and 5′-flanking sequences. The plasmids so constructed were tested for ability to restore UV-resistant phenotype to uvrC-; cells as an indication of expression of the uvrC gene. The chromosomal DNA in plasmid pUV7 was probed for strong binding with E. coli RNA polymerase in an attempt to identify a restriction fragment which bears the regulatory sequences for the uvrC transcriptional unit. The results indicate that DNA sequences at least 0.9 Kb upstream from the structural gene, but not the 5′-proximal sequences, regulate expression of the uvrC gene. Analysis of protein synthesis encoded by plasmid pUV7 and its derivatives suggest that there may be another gene that lies between the promoter and the uvrC gene and codes for a 27,000-Mr protein. The relation of this gene to uvrC function is not clear.

Original languageEnglish (US)
Pages (from-to)5209-5221
Number of pages13
JournalNucleic Acids Research
Volume10
Issue number17
DOIs
StatePublished - Sep 11 1982
Externally publishedYes

Fingerprint

DNA sequences
Transcription
Molecular Structure
DNA Sequence
Escherichia coli
Molecular structure
Escherichia Coli
Genes
Gene
Plasmids
Gene Expression
5' Flanking Region
Nucleic Acid Regulatory Sequences
Proteins
DNA-Directed RNA Polymerases
Unit
Protein Synthesis
RNA
Promoter
Phenotype

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Molecular structure of the uvrC gene of Escherichia coli : Identification of DNA sequences required for transcription of the uvrC gene. / Sharma, Surendra; Dowhan, William; Moses, Robb.

In: Nucleic Acids Research, Vol. 10, No. 17, 11.09.1982, p. 5209-5221.

Research output: Contribution to journalArticle

@article{5875b063ce184b178a609230546dbc7c,
title = "Molecular structure of the uvrC gene of Escherichia coli: Identification of DNA sequences required for transcription of the uvrC gene",
abstract = "We have carried out experiments to identify the regulatory regions of the uvrC gene of Escherichia coli. A uvrC+ plasmid, pUV7, containing the intact transcriptional unit for the uvrC gene, was used to subclone either the structural gene or combinations of the structural gene and 5′-flanking sequences. The plasmids so constructed were tested for ability to restore UV-resistant phenotype to uvrC-; cells as an indication of expression of the uvrC gene. The chromosomal DNA in plasmid pUV7 was probed for strong binding with E. coli RNA polymerase in an attempt to identify a restriction fragment which bears the regulatory sequences for the uvrC transcriptional unit. The results indicate that DNA sequences at least 0.9 Kb upstream from the structural gene, but not the 5′-proximal sequences, regulate expression of the uvrC gene. Analysis of protein synthesis encoded by plasmid pUV7 and its derivatives suggest that there may be another gene that lies between the promoter and the uvrC gene and codes for a 27,000-Mr protein. The relation of this gene to uvrC function is not clear.",
author = "Surendra Sharma and William Dowhan and Robb Moses",
year = "1982",
month = "9",
day = "11",
doi = "10.1093/nar/10.17.5209",
language = "English (US)",
volume = "10",
pages = "5209--5221",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "17",

}

TY - JOUR

T1 - Molecular structure of the uvrC gene of Escherichia coli

T2 - Identification of DNA sequences required for transcription of the uvrC gene

AU - Sharma, Surendra

AU - Dowhan, William

AU - Moses, Robb

PY - 1982/9/11

Y1 - 1982/9/11

N2 - We have carried out experiments to identify the regulatory regions of the uvrC gene of Escherichia coli. A uvrC+ plasmid, pUV7, containing the intact transcriptional unit for the uvrC gene, was used to subclone either the structural gene or combinations of the structural gene and 5′-flanking sequences. The plasmids so constructed were tested for ability to restore UV-resistant phenotype to uvrC-; cells as an indication of expression of the uvrC gene. The chromosomal DNA in plasmid pUV7 was probed for strong binding with E. coli RNA polymerase in an attempt to identify a restriction fragment which bears the regulatory sequences for the uvrC transcriptional unit. The results indicate that DNA sequences at least 0.9 Kb upstream from the structural gene, but not the 5′-proximal sequences, regulate expression of the uvrC gene. Analysis of protein synthesis encoded by plasmid pUV7 and its derivatives suggest that there may be another gene that lies between the promoter and the uvrC gene and codes for a 27,000-Mr protein. The relation of this gene to uvrC function is not clear.

AB - We have carried out experiments to identify the regulatory regions of the uvrC gene of Escherichia coli. A uvrC+ plasmid, pUV7, containing the intact transcriptional unit for the uvrC gene, was used to subclone either the structural gene or combinations of the structural gene and 5′-flanking sequences. The plasmids so constructed were tested for ability to restore UV-resistant phenotype to uvrC-; cells as an indication of expression of the uvrC gene. The chromosomal DNA in plasmid pUV7 was probed for strong binding with E. coli RNA polymerase in an attempt to identify a restriction fragment which bears the regulatory sequences for the uvrC transcriptional unit. The results indicate that DNA sequences at least 0.9 Kb upstream from the structural gene, but not the 5′-proximal sequences, regulate expression of the uvrC gene. Analysis of protein synthesis encoded by plasmid pUV7 and its derivatives suggest that there may be another gene that lies between the promoter and the uvrC gene and codes for a 27,000-Mr protein. The relation of this gene to uvrC function is not clear.

UR - http://www.scopus.com/inward/record.url?scp=0020480507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020480507&partnerID=8YFLogxK

U2 - 10.1093/nar/10.17.5209

DO - 10.1093/nar/10.17.5209

M3 - Article

VL - 10

SP - 5209

EP - 5221

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 17

ER -