Molecular MR imaging of liver fibrosis

A feasibility study using rat and mouse models

Miloslav Polasek, Bryan C. Fuchs, Ritika Uppal, Daniel T. Schühle, Jamu K. Alford, Galen S. Loving, Suguru Yamada, Lan Wei, Gregory Y. Lauwers, Alexander Guimaraes, Kenneth K. Tanabe, Peter Caravan

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Background & Aims: Liver biopsy, the current clinical gold standard for fibrosis assessment, is invasive and has sampling errors, and is not optimal for screening, monitoring, or clinical decision-making. Fibrosis is characterized by excessive accumulation of extracellular matrix proteins including type I collagen. We hypothesize that molecular magnetic resonance imaging (MRI) with a probe targeted to type I collagen could provide a direct and non-invasive method of fibrosis assessment. Methods: Liver fibrosis was induced in rats with diethylnitrosamine and in mice with carbon tetrachloride. Animals were imaged prior to and immediately following i.v. administration of either collagen-targeted probe EP-3533 or non-targeted control Gd-DTPA. Magnetic resonance (MR) signal washout characteristics were evaluated from T1 maps and T1-weighted images. Liver tissue was subjected to pathologic scoring of fibrosis and analyzed for gadolinium and hydroxyproline. Results: EP-3533-enhanced MR showed greater signal intensity on delayed imaging (normalized signal enhancement mice: control = 0.39 ± 0.04, fibrotic = 0.55 ± 0.03, p 1/2 = 51.3 ± 3.6 vs. 42.0 ± 2.5 min, p

Original languageEnglish (US)
Pages (from-to)549-555
Number of pages7
JournalJournal of Hepatology
Volume57
Issue number3
DOIs
StatePublished - Sep 2012
Externally publishedYes

Fingerprint

Feasibility Studies
Liver Cirrhosis
Fibrosis
Magnetic Resonance Imaging
Collagen Type I
Magnetic Resonance Spectroscopy
Diethylnitrosamine
Gadolinium DTPA
Selection Bias
Carbon Tetrachloride
Extracellular Matrix Proteins
Liver
Hydroxyproline
Gadolinium
Collagen
Biopsy

Keywords

  • Carbon tetrachloride
  • Diethylnitrosamine
  • Gadolinium
  • Molecular imaging
  • Type I collagen

ASJC Scopus subject areas

  • Hepatology

Cite this

Polasek, M., Fuchs, B. C., Uppal, R., Schühle, D. T., Alford, J. K., Loving, G. S., ... Caravan, P. (2012). Molecular MR imaging of liver fibrosis: A feasibility study using rat and mouse models. Journal of Hepatology, 57(3), 549-555. https://doi.org/10.1016/j.jhep.2012.04.035

Molecular MR imaging of liver fibrosis : A feasibility study using rat and mouse models. / Polasek, Miloslav; Fuchs, Bryan C.; Uppal, Ritika; Schühle, Daniel T.; Alford, Jamu K.; Loving, Galen S.; Yamada, Suguru; Wei, Lan; Lauwers, Gregory Y.; Guimaraes, Alexander; Tanabe, Kenneth K.; Caravan, Peter.

In: Journal of Hepatology, Vol. 57, No. 3, 09.2012, p. 549-555.

Research output: Contribution to journalArticle

Polasek, M, Fuchs, BC, Uppal, R, Schühle, DT, Alford, JK, Loving, GS, Yamada, S, Wei, L, Lauwers, GY, Guimaraes, A, Tanabe, KK & Caravan, P 2012, 'Molecular MR imaging of liver fibrosis: A feasibility study using rat and mouse models', Journal of Hepatology, vol. 57, no. 3, pp. 549-555. https://doi.org/10.1016/j.jhep.2012.04.035
Polasek, Miloslav ; Fuchs, Bryan C. ; Uppal, Ritika ; Schühle, Daniel T. ; Alford, Jamu K. ; Loving, Galen S. ; Yamada, Suguru ; Wei, Lan ; Lauwers, Gregory Y. ; Guimaraes, Alexander ; Tanabe, Kenneth K. ; Caravan, Peter. / Molecular MR imaging of liver fibrosis : A feasibility study using rat and mouse models. In: Journal of Hepatology. 2012 ; Vol. 57, No. 3. pp. 549-555.
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