Molecular imaging of the initial inflammatory response in atherosclerosis

Implications for early detection of disease

Beat A. Kaufmann, Chad L. Carr, J. Todd Belcik, Aris Xie, Qi Yue, Scott Chadderdon, Evan S. Caplan, Jaspreet Khangura, Sherry Bullens, Stuart Bunting, Jonathan Lindner

    Research output: Contribution to journalArticle

    129 Citations (Scopus)

    Abstract

    BACKGROUND-: We hypothesized that molecular imaging of endothelial cell adhesion molecule expression could noninvasively evaluate prelesion atherogenic phenotype. METHODS AND RESULTS-: Mice deficient for the LDL-receptor and the Apobec-1 editing peptide (DKO mice) were studied as an age-dependent model of atherosclerosis. At 10, 20, and 40 weeks of age, ultrasound molecular imaging of the proximal thoracic aorta was performed with contrast agents targeted to P-selectin and VCAM-1. Atherosclerotic lesion severity and content were assessed by ultrahigh frequency ultrasound, histology, and immunohistochemistry. In wild-type mice at all ages, there was neither aortic thickening nor targeted tracer signal enhancement. In DKO mice, lesions progressed from sparse mild intimal thickening at 10 weeks to widespread severe lesions with luminal encroachment at 40 weeks. Molecular imaging for P-selectin and VCAM-1 demonstrated selective signal enhancement (P

    Original languageEnglish (US)
    Pages (from-to)54-59
    Number of pages6
    JournalArteriosclerosis, Thrombosis, and Vascular Biology
    Volume30
    Issue number1
    DOIs
    StatePublished - Jan 2010

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    Molecular Imaging
    Early Diagnosis
    Atherosclerosis
    P-Selectin
    Vascular Cell Adhesion Molecule-1
    Tunica Intima
    LDL Receptors
    Cell Adhesion Molecules
    Thoracic Aorta
    Contrast Media
    Ultrasonography
    Histology
    Endothelial Cells
    Immunohistochemistry
    Phenotype
    Peptides

    Keywords

    • Atherosclerosis
    • Microbubbles
    • Molecular imaging
    • P-selectin
    • VCAA-1

    ASJC Scopus subject areas

    • Cardiology and Cardiovascular Medicine

    Cite this

    Molecular imaging of the initial inflammatory response in atherosclerosis : Implications for early detection of disease. / Kaufmann, Beat A.; Carr, Chad L.; Belcik, J. Todd; Xie, Aris; Yue, Qi; Chadderdon, Scott; Caplan, Evan S.; Khangura, Jaspreet; Bullens, Sherry; Bunting, Stuart; Lindner, Jonathan.

    In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 30, No. 1, 01.2010, p. 54-59.

    Research output: Contribution to journalArticle

    Kaufmann, Beat A. ; Carr, Chad L. ; Belcik, J. Todd ; Xie, Aris ; Yue, Qi ; Chadderdon, Scott ; Caplan, Evan S. ; Khangura, Jaspreet ; Bullens, Sherry ; Bunting, Stuart ; Lindner, Jonathan. / Molecular imaging of the initial inflammatory response in atherosclerosis : Implications for early detection of disease. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2010 ; Vol. 30, No. 1. pp. 54-59.
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