Molecular imaging of platelet-endothelial interactions and endothelial von Willebrand factor in early and mid-stage atherosclerosis

Chi Young Shim, Ya Ni Liu, Tamara (Tami) Atkinson, Aris Xie, Ted Foster, Brian P. Davidson, Mackenzie Treible, Qi Yue, José A. López, Adam Munday, Zaverio Ruggeri, Jonathan Lindner

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26 Scopus citations

Abstract

Background: Nonthrombotic platelet-endothelial interactions may contribute to atherosclerotic plaque development, although in vivo studies examining mechanism without platelet preactivation are lacking. Using in vivo molecular imaging at various stages of atherosclerosis, we quantified platelet-endothelial interactions and evaluated the contribution of major adhesion pathways. Methods and Results: Mice deficient for the low-density lipoprotein receptor and Apobec-1 were studied as an agedependent model of atherosclerosis at 10, 20, 30, and 40 weeks of age, which provided progressive increase in stage from early fatty streak (10 weeks) to large complex plaques without rupture (40 weeks). Platelet-targeted contrast ultrasound molecular imaging of the thoracic aorta performed with microbubbles targeted to GPIbá demonstrated selective signal enhancement as early as 10 weeks of age. This signal increased progressively with age (almost 8-fold increase from 10 to 40 weeks, analysis of variance P

Original languageEnglish (US)
Article numbere002765
JournalCirculation: Cardiovascular Imaging
Volume8
Issue number7
DOIs
Publication statusPublished - May 1 2015

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Keywords

  • Atherosclerosis
  • Molecular imaging
  • Platelet
  • Von Willebrand factor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Radiology Nuclear Medicine and imaging

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