Molecular imaging of endothelial vascular cell adhesion molecule-1 expression and inflammatory cell recruitment during vasculogenesis and ischemia-mediated arteriogenesis

Carolyn Z. Behm, Beat A. Kaufmann, Chad Carr, Miles Lankford, John M. Sanders, C. Edward Rose, Sanjiv Kaul, Jonathan Lindner

    Research output: Contribution to journalArticle

    99 Citations (Scopus)

    Abstract

    BACKGROUND - Inflammatory responses contribute to vascular remodeling during tissue repair or ischemia. We hypothesized that inflammatory cell recruitment and endothelial cell activation during vasculogenesis and ischemia-mediated arteriogenesis could be temporally assessed by noninvasive molecular imaging. METHODS AND RESULTS - Contrast ultrasound perfusion imaging and molecular imaging with microbubbles targeted to activated neutrophils, α5-integrins, or vascular cell adhesion molecule (VCAM-1) were performed in murine models of vasculogenesis (subcutaneous matrigel) or hind-limb ischemia produced by arterial occlusion in wild-type or monocyte chemotactic protein-1-deficient mice. In subcutaneous matrigel plugs, perfusion advanced centripetally between days 3 and 10. On targeted imaging, signal enhancement from α5-integrins and VCAM-1 coincided with the earliest appearance of regional blood flow. Targeted imaging correlated temporally with histological evidence of channel formation by α5-integrin-positive monocytes, followed by the appearance of spindle-shaped cells lining the channels that expressed VCAM-1. In ischemic hind-limb tissue, skeletal muscle blood flow and arteriolar density increased progressively between days 2 and 21 after arterial ligation. Targeted imaging demonstrated early signal enhancement for neutrophils, monocyte α5-integrin, and VCAM-1 at day 2 when blood flow was very low (

    Original languageEnglish (US)
    Pages (from-to)2902-2911
    Number of pages10
    JournalCirculation
    Volume117
    Issue number22
    DOIs
    StatePublished - Jun 2008

    Fingerprint

    Molecular Imaging
    Vascular Cell Adhesion Molecule-1
    Ischemia
    Integrins
    Monocytes
    Neutrophils
    Extremities
    Microbubbles
    Perfusion Imaging
    Chemokine CCL2
    Regional Blood Flow
    Ligation
    Ultrasonography
    Skeletal Muscle
    Endothelial Cells
    Perfusion

    Keywords

    • Angiogenesis
    • Echocardiography
    • Imaging
    • Inflammation

    ASJC Scopus subject areas

    • Physiology
    • Cardiology and Cardiovascular Medicine

    Cite this

    Molecular imaging of endothelial vascular cell adhesion molecule-1 expression and inflammatory cell recruitment during vasculogenesis and ischemia-mediated arteriogenesis. / Behm, Carolyn Z.; Kaufmann, Beat A.; Carr, Chad; Lankford, Miles; Sanders, John M.; Rose, C. Edward; Kaul, Sanjiv; Lindner, Jonathan.

    In: Circulation, Vol. 117, No. 22, 06.2008, p. 2902-2911.

    Research output: Contribution to journalArticle

    Behm, Carolyn Z. ; Kaufmann, Beat A. ; Carr, Chad ; Lankford, Miles ; Sanders, John M. ; Rose, C. Edward ; Kaul, Sanjiv ; Lindner, Jonathan. / Molecular imaging of endothelial vascular cell adhesion molecule-1 expression and inflammatory cell recruitment during vasculogenesis and ischemia-mediated arteriogenesis. In: Circulation. 2008 ; Vol. 117, No. 22. pp. 2902-2911.
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    AU - Sanders, John M.

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