Abstract
Low back pain is a prevalent medical condition that is difficult to diagnose and treat. Current imaging methods are unable to correlate pain reliably with spinal structures, and surgical removal of painful damaged or degenerating disks is technically challenging. A contrast agent specific for the intervertebral disk could assist in the detection, diagnosis, and surgical treatment of low back pain. The styryl pyridinium (FM) fluorophores were characterized and structure-activity relationships between chemical structure and in vivo uptake were established. Two novel FM fluorophores with improved optical properties for imaging the intervertebral disks were synthesized and evaluated in mice, rats, and pigs. After a single systemic injection, eight of eight FM fluorophores provided high-contrast imaging of the trigeminal ganglia, whereas six of eight provided high-contrast imaging of the dorsal root ganglia. Unexpectedly, three of eight FM fluorophores provided high-contrast imaging of annulus fibrosus tissue of the intervertebral disks, confirmed histologically. We present the first known contrast agent specific for the intervertebral disks and identify the chemical structural motif that mediates uptake. FM fluorophores could be used for image-guided surgery to assist in the removal of intervertebral disk and lay the foundation for derivatives for magnetic resonance imaging and positron emission tomography.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 128-140 |
| Number of pages | 13 |
| Journal | Molecular Imaging |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Biotechnology
- Molecular Medicine
- Biomedical Engineering
- Condensed Matter Physics
- Medicine(all)
Cite this
Molecular imaging agents specific for the annulus fibrosus of the intervertebral disk. / Gibbs, Summer; Vooght, Carrie; Fish, Kenneth M.; Nasr, Khaled A.; Siclovan, Tiberiu M.; Barnhardt, Nicole E.; Hehir, Cristina A Tan; Frangioni, John V.
In: Molecular Imaging, Vol. 9, No. 3, 05.2010, p. 128-140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular imaging agents specific for the annulus fibrosus of the intervertebral disk
AU - Gibbs, Summer
AU - Vooght, Carrie
AU - Fish, Kenneth M.
AU - Nasr, Khaled A.
AU - Siclovan, Tiberiu M.
AU - Barnhardt, Nicole E.
AU - Hehir, Cristina A Tan
AU - Frangioni, John V.
PY - 2010/5
Y1 - 2010/5
N2 - Low back pain is a prevalent medical condition that is difficult to diagnose and treat. Current imaging methods are unable to correlate pain reliably with spinal structures, and surgical removal of painful damaged or degenerating disks is technically challenging. A contrast agent specific for the intervertebral disk could assist in the detection, diagnosis, and surgical treatment of low back pain. The styryl pyridinium (FM) fluorophores were characterized and structure-activity relationships between chemical structure and in vivo uptake were established. Two novel FM fluorophores with improved optical properties for imaging the intervertebral disks were synthesized and evaluated in mice, rats, and pigs. After a single systemic injection, eight of eight FM fluorophores provided high-contrast imaging of the trigeminal ganglia, whereas six of eight provided high-contrast imaging of the dorsal root ganglia. Unexpectedly, three of eight FM fluorophores provided high-contrast imaging of annulus fibrosus tissue of the intervertebral disks, confirmed histologically. We present the first known contrast agent specific for the intervertebral disks and identify the chemical structural motif that mediates uptake. FM fluorophores could be used for image-guided surgery to assist in the removal of intervertebral disk and lay the foundation for derivatives for magnetic resonance imaging and positron emission tomography.
AB - Low back pain is a prevalent medical condition that is difficult to diagnose and treat. Current imaging methods are unable to correlate pain reliably with spinal structures, and surgical removal of painful damaged or degenerating disks is technically challenging. A contrast agent specific for the intervertebral disk could assist in the detection, diagnosis, and surgical treatment of low back pain. The styryl pyridinium (FM) fluorophores were characterized and structure-activity relationships between chemical structure and in vivo uptake were established. Two novel FM fluorophores with improved optical properties for imaging the intervertebral disks were synthesized and evaluated in mice, rats, and pigs. After a single systemic injection, eight of eight FM fluorophores provided high-contrast imaging of the trigeminal ganglia, whereas six of eight provided high-contrast imaging of the dorsal root ganglia. Unexpectedly, three of eight FM fluorophores provided high-contrast imaging of annulus fibrosus tissue of the intervertebral disks, confirmed histologically. We present the first known contrast agent specific for the intervertebral disks and identify the chemical structural motif that mediates uptake. FM fluorophores could be used for image-guided surgery to assist in the removal of intervertebral disk and lay the foundation for derivatives for magnetic resonance imaging and positron emission tomography.
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UR - http://www.scopus.com/inward/citedby.url?scp=77953519433&partnerID=8YFLogxK
U2 - 10.2310/7290.2010.00009
DO - 10.2310/7290.2010.00009
M3 - Article
C2 - 20487679
AN - SCOPUS:77953519433
VL - 9
SP - 128
EP - 140
JO - Molecular imaging : official journal of the Society for Molecular Imaging
JF - Molecular imaging : official journal of the Society for Molecular Imaging
SN - 1535-3508
IS - 3
ER -