Molecular imaging agents specific for the annulus fibrosus of the intervertebral disk

Summer Gibbs, Carrie Vooght, Kenneth M. Fish, Khaled A. Nasr, Tiberiu M. Siclovan, Nicole E. Barnhardt, Cristina A Tan Hehir, John V. Frangioni

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Low back pain is a prevalent medical condition that is difficult to diagnose and treat. Current imaging methods are unable to correlate pain reliably with spinal structures, and surgical removal of painful damaged or degenerating disks is technically challenging. A contrast agent specific for the intervertebral disk could assist in the detection, diagnosis, and surgical treatment of low back pain. The styryl pyridinium (FM) fluorophores were characterized and structure-activity relationships between chemical structure and in vivo uptake were established. Two novel FM fluorophores with improved optical properties for imaging the intervertebral disks were synthesized and evaluated in mice, rats, and pigs. After a single systemic injection, eight of eight FM fluorophores provided high-contrast imaging of the trigeminal ganglia, whereas six of eight provided high-contrast imaging of the dorsal root ganglia. Unexpectedly, three of eight FM fluorophores provided high-contrast imaging of annulus fibrosus tissue of the intervertebral disks, confirmed histologically. We present the first known contrast agent specific for the intervertebral disks and identify the chemical structural motif that mediates uptake. FM fluorophores could be used for image-guided surgery to assist in the removal of intervertebral disk and lay the foundation for derivatives for magnetic resonance imaging and positron emission tomography.

Original languageEnglish (US)
Pages (from-to)128-140
Number of pages13
JournalMolecular Imaging
Volume9
Issue number3
DOIs
StatePublished - May 2010
Externally publishedYes

Fingerprint

intervertebral disks
Molecular imaging
Molecular Imaging
Intervertebral Disc
annuli
Fluorophores
frequency modulation
pain
Imaging techniques
Low Back Pain
Contrast Media
Computer-Assisted Surgery
Trigeminal Ganglion
swine
Optical Imaging
Spinal Ganglia
Structure-Activity Relationship
Positron emission tomography
surgery
Positron-Emission Tomography

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biotechnology
  • Molecular Medicine
  • Biomedical Engineering
  • Condensed Matter Physics
  • Medicine(all)

Cite this

Gibbs, S., Vooght, C., Fish, K. M., Nasr, K. A., Siclovan, T. M., Barnhardt, N. E., ... Frangioni, J. V. (2010). Molecular imaging agents specific for the annulus fibrosus of the intervertebral disk. Molecular Imaging, 9(3), 128-140. https://doi.org/10.2310/7290.2010.00009

Molecular imaging agents specific for the annulus fibrosus of the intervertebral disk. / Gibbs, Summer; Vooght, Carrie; Fish, Kenneth M.; Nasr, Khaled A.; Siclovan, Tiberiu M.; Barnhardt, Nicole E.; Hehir, Cristina A Tan; Frangioni, John V.

In: Molecular Imaging, Vol. 9, No. 3, 05.2010, p. 128-140.

Research output: Contribution to journalArticle

Gibbs, S, Vooght, C, Fish, KM, Nasr, KA, Siclovan, TM, Barnhardt, NE, Hehir, CAT & Frangioni, JV 2010, 'Molecular imaging agents specific for the annulus fibrosus of the intervertebral disk', Molecular Imaging, vol. 9, no. 3, pp. 128-140. https://doi.org/10.2310/7290.2010.00009
Gibbs, Summer ; Vooght, Carrie ; Fish, Kenneth M. ; Nasr, Khaled A. ; Siclovan, Tiberiu M. ; Barnhardt, Nicole E. ; Hehir, Cristina A Tan ; Frangioni, John V. / Molecular imaging agents specific for the annulus fibrosus of the intervertebral disk. In: Molecular Imaging. 2010 ; Vol. 9, No. 3. pp. 128-140.
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