Molecular genetic analysis of the Escherichia coli phoP locus

E. A. Groisman, Fred Heffron, F. Solomon

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We have cloned the Escherichia coli phoP gene, a member of the family of environmentally responsive two-component systems, and found its deduced amino acid sequence to be 93% identical to that of the Salmonella typhimurium homolog, which encodes a major virulence regulator necessary for intramacrophage survival and resistance to cationic peptides of phagocytic cells. The phoP gene was mapped to kilobase 1202 on the Kohara map (25-min region) of the E. coli genome (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987) and found to be transcribed in a counterclockwise direction. Both E. coli and S. typhimurium phoP mutants were more sensitive than their isogenic wild-type strains to the frog-derived antibacterial peptide magainin 2, suggesting a role for PhoP in the response to various stresses in both enteric species.

Original languageEnglish (US)
Pages (from-to)486-491
Number of pages6
JournalJournal of Bacteriology
Volume174
Issue number2
StatePublished - 1992
Externally publishedYes

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Molecular Biology
Salmonella typhimurium
Escherichia coli
Magainins
Peptides
Phagocytes
Anura
Genes
Virulence
Amino Acid Sequence
Genome
Direction compound

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Groisman, E. A., Heffron, F., & Solomon, F. (1992). Molecular genetic analysis of the Escherichia coli phoP locus. Journal of Bacteriology, 174(2), 486-491.

Molecular genetic analysis of the Escherichia coli phoP locus. / Groisman, E. A.; Heffron, Fred; Solomon, F.

In: Journal of Bacteriology, Vol. 174, No. 2, 1992, p. 486-491.

Research output: Contribution to journalArticle

Groisman, EA, Heffron, F & Solomon, F 1992, 'Molecular genetic analysis of the Escherichia coli phoP locus', Journal of Bacteriology, vol. 174, no. 2, pp. 486-491.
Groisman EA, Heffron F, Solomon F. Molecular genetic analysis of the Escherichia coli phoP locus. Journal of Bacteriology. 1992;174(2):486-491.
Groisman, E. A. ; Heffron, Fred ; Solomon, F. / Molecular genetic analysis of the Escherichia coli phoP locus. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 2. pp. 486-491.
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