Molecular genetic analysis of myoF, a new Dictyostelium myosin I gene

M. A. Titus, K. D. Novak, G. P. Hanes, A. S. Urioste, R. Chisholm, M. Ostap, R. Cooke, Peter Barr-Gillespie, S. Block

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Several new members of the Dictyostelium myosin family have been identified by physical mapping techniques in combination with PCR. Here we describe the initial molecular genetic characterization of one of these, myoF. A 1-kb segment of the myoF gene was obtained by the PCR and used as a specific probe for Northern analysis and as a vehicle for gene-targeting studies. The myoF gene is expressed as a 3.7-kb message, a size consistent with it encoding a myosin I class unconventional myosin, bringing the total of myosin is present in Dictyostelium to six. Analysis of strains in which the myoF gene has been disrupted reveals that loss of the myoF protein does not result in obvious defects either in cellular translocation, or in other readily assayed actin-based processes. The results of our investigation indicate that the myosin I family is quite large in Dictyostelium, and that several members, including myoF, may either be functionally redundant or play roles in as yet undescribed actin-based processes.

Original languageEnglish (US)
JournalBiophysical Journal
Volume68
Issue number4 SUPPL.
StatePublished - 1995
Externally publishedYes

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Myosin Type I
Dictyostelium
Myosins
Molecular Biology
Actins
Genes
Polymerase Chain Reaction
Gene Targeting
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Titus, M. A., Novak, K. D., Hanes, G. P., Urioste, A. S., Chisholm, R., Ostap, M., ... Block, S. (1995). Molecular genetic analysis of myoF, a new Dictyostelium myosin I gene. Biophysical Journal, 68(4 SUPPL.).

Molecular genetic analysis of myoF, a new Dictyostelium myosin I gene. / Titus, M. A.; Novak, K. D.; Hanes, G. P.; Urioste, A. S.; Chisholm, R.; Ostap, M.; Cooke, R.; Barr-Gillespie, Peter; Block, S.

In: Biophysical Journal, Vol. 68, No. 4 SUPPL., 1995.

Research output: Contribution to journalArticle

Titus, MA, Novak, KD, Hanes, GP, Urioste, AS, Chisholm, R, Ostap, M, Cooke, R, Barr-Gillespie, P & Block, S 1995, 'Molecular genetic analysis of myoF, a new Dictyostelium myosin I gene', Biophysical Journal, vol. 68, no. 4 SUPPL..
Titus MA, Novak KD, Hanes GP, Urioste AS, Chisholm R, Ostap M et al. Molecular genetic analysis of myoF, a new Dictyostelium myosin I gene. Biophysical Journal. 1995;68(4 SUPPL.).
Titus, M. A. ; Novak, K. D. ; Hanes, G. P. ; Urioste, A. S. ; Chisholm, R. ; Ostap, M. ; Cooke, R. ; Barr-Gillespie, Peter ; Block, S. / Molecular genetic analysis of myoF, a new Dictyostelium myosin I gene. In: Biophysical Journal. 1995 ; Vol. 68, No. 4 SUPPL.
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