Molecular epidemiology of dual HIV-1/HIV-2 seropositive adults from Senegal, West Africa

Geoffrey S. Gottlieb, Papa Salif Sow, Stephen E. Hawes, Ibra Ndoye, Awa M. Coll-Seck, Marcel E. Curlin, Cathy W. Critchlow, Nancy B. Kiviat, James I. Mullins

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Dual infection with HIV-1 and HIV-2 can occur in locales where these viruses co-circulate, most commonly in West Africa. Although dual seropositivity is common in this region, the true rate of dual infection remains unclear. In addition, whether unique HIV-1 subtypes are circulating in dually infected individuals is unknown. A cohort of 47 HIV-1 and HIV-2 dually seropositive individuals from Senegal, West Africa was screened for the presence of HIV-1 and HIV-2 gag and env PBMC viral DNA sequences using PCR. Of the 47 dual HIV-1/HIV-2 seropositive individuals tested, 19 (40.4%) had infection with both HIV-1 and HIV-2 Confirmed by genetic sequence analysis, whereas only HIV-1 or HIV-2 was confirmed in 17 (36.2%) or 9 (19.1%), respectively. The majority of HIV-1 subtypes found were CRF-02 and A, although subtypes D, C, G, J and B were also found, reflecting the subtypes known to be circulating in Senegal. There was no significant difference in HIV-1 subtype distribution between individuals with confirmed dual infection and patients in this study with dual seropositivity but lacking HIV-2, or with HIV-1 infected patients within the general population in Senegal, although the study was underpowered to detect anything but large differences. The prevalence of HIV-1/HIV-2 dual infection appears to be significantly less than that of dually seropositive individuals and this likely reflects cross-reactive serology. The common HIV-1 subtypes prevalent in West Africa (CRF-02 and subtype A) have a similar distribution to those found in our cohort of dually infected and dually seropositive subjects.

Original languageEnglish (US)
Pages (from-to)575-584
Number of pages10
JournalAIDS Research and Human Retroviruses
Volume19
Issue number7
DOIs
StatePublished - Jul 1 2003

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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    Gottlieb, G. S., Sow, P. S., Hawes, S. E., Ndoye, I., Coll-Seck, A. M., Curlin, M. E., Critchlow, C. W., Kiviat, N. B., & Mullins, J. I. (2003). Molecular epidemiology of dual HIV-1/HIV-2 seropositive adults from Senegal, West Africa. AIDS Research and Human Retroviruses, 19(7), 575-584. https://doi.org/10.1089/088922203322230941