Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain

Arpiar Saunders; Evan Z. Macosko; Alec Wysoker; Melissa Goldman; Fenna M. Krienen; Heather de Rivera; Elizabeth Bien; Matthew Baum; Laura Bortolin; Shuyu Wang; Aleksandrina Goeva; James Nemesh; Nolan Kamitaki; Sara Brumbaugh; David Kulp; Steven A. McCarroll

doi:10.1016/j.cell.2018.07.028

Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain

Arpiar Saunders, Evan Z. Macosko, Alec Wysoker, Melissa Goldman, Fenna M. Krienen, Heather de Rivera, Elizabeth Bien, Matthew Baum, Laura Bortolin, Shuyu Wang, Aleksandrina Goeva, James Nemesh, Nolan Kamitaki, Sara Brumbaugh, David Kulp, Steven A. McCarroll

Research output: Contribution to journal › Article › peer-review

852 Scopus citations

Abstract

The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution. Sampling across multiple brain regions identifies hundreds of transcriptionally distinct groups of cells and reveals large-scale features of brain organization and neuronal diversity.

Original language	English (US)
Pages (from-to)	1015-1030.e16
Journal	Cell
Volume	174
Issue number	4
DOIs	https://doi.org/10.1016/j.cell.2018.07.028
State	Published - Aug 9 2018
Externally published	Yes

Keywords

basal ganglia
brain
single-cell
striatum
transcriptional programs

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2018.07.028

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Saunders, A., Macosko, E. Z., Wysoker, A., Goldman, M., Krienen, F. M., de Rivera, H., Bien, E., Baum, M., Bortolin, L., Wang, S., Goeva, A., Nemesh, J., Kamitaki, N., Brumbaugh, S., Kulp, D., & McCarroll, S. A. (2018). Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain. Cell, 174(4), 1015-1030.e16. https://doi.org/10.1016/j.cell.2018.07.028

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abstract = "The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution. Sampling across multiple brain regions identifies hundreds of transcriptionally distinct groups of cells and reveals large-scale features of brain organization and neuronal diversity.",

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doi = "10.1016/j.cell.2018.07.028",

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AU - de Rivera, Heather

AU - Bien, Elizabeth

AU - Baum, Matthew

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AU - Wang, Shuyu

AU - Goeva, Aleksandrina

AU - Nemesh, James

AU - Kamitaki, Nolan

AU - Brumbaugh, Sara

AU - Kulp, David

AU - McCarroll, Steven A.

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N2 - The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution. Sampling across multiple brain regions identifies hundreds of transcriptionally distinct groups of cells and reveals large-scale features of brain organization and neuronal diversity.

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Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain

Abstract

Keywords

ASJC Scopus subject areas

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