Abstract
Acute leukemia represents one of the best-studied malignancies. Consequently, numerous dogmas have evolved over the years. One dogma to which we subscribe is that leukemia is a genetic disease and that the behavior of leukemic cells and their response to therapy is determined largely by their genetic characteristics. The relative ease of metaphase generation and culture of single leukemic cells, well-defined morphologic criteria, and availability of phenotyping reagents has contributed to a wealth of information about the karyotypic, genetic, and functional properties of these cells. Nevertheless, establishment of genotype-phenotype relationships has been particularly elusive. The genetic evolution of leukemias and the consequences of specific aberrations remains to be determined. We describe the power of new molecular cytogenetic tools, fluorescence in situ hybridization, and comparative genomic hybridization to measure the genetic evolution of leukemic cells. Lessons learned from application of these techniques to solid tumors and speculations about similar approaches to analysis of leukemia are addressed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 677-683 |
| Number of pages | 7 |
| Journal | Blood Cells |
| Volume | 19 |
| Issue number | 3 |
| State | Published - 1993 |
| Externally published | Yes |
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Keywords
- leukemia
- molecular cytogenetics
- solid tumors
ASJC Scopus subject areas
- Hematology
Cite this
Molecular cytogenetics : Solid tumors and leukemia. / Gray, Joe; Pallavicini, M. G.
In: Blood Cells, Vol. 19, No. 3, 1993, p. 677-683.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cytogenetics
T2 - Solid tumors and leukemia
AU - Gray, Joe
AU - Pallavicini, M. G.
PY - 1993
Y1 - 1993
N2 - Acute leukemia represents one of the best-studied malignancies. Consequently, numerous dogmas have evolved over the years. One dogma to which we subscribe is that leukemia is a genetic disease and that the behavior of leukemic cells and their response to therapy is determined largely by their genetic characteristics. The relative ease of metaphase generation and culture of single leukemic cells, well-defined morphologic criteria, and availability of phenotyping reagents has contributed to a wealth of information about the karyotypic, genetic, and functional properties of these cells. Nevertheless, establishment of genotype-phenotype relationships has been particularly elusive. The genetic evolution of leukemias and the consequences of specific aberrations remains to be determined. We describe the power of new molecular cytogenetic tools, fluorescence in situ hybridization, and comparative genomic hybridization to measure the genetic evolution of leukemic cells. Lessons learned from application of these techniques to solid tumors and speculations about similar approaches to analysis of leukemia are addressed.
AB - Acute leukemia represents one of the best-studied malignancies. Consequently, numerous dogmas have evolved over the years. One dogma to which we subscribe is that leukemia is a genetic disease and that the behavior of leukemic cells and their response to therapy is determined largely by their genetic characteristics. The relative ease of metaphase generation and culture of single leukemic cells, well-defined morphologic criteria, and availability of phenotyping reagents has contributed to a wealth of information about the karyotypic, genetic, and functional properties of these cells. Nevertheless, establishment of genotype-phenotype relationships has been particularly elusive. The genetic evolution of leukemias and the consequences of specific aberrations remains to be determined. We describe the power of new molecular cytogenetic tools, fluorescence in situ hybridization, and comparative genomic hybridization to measure the genetic evolution of leukemic cells. Lessons learned from application of these techniques to solid tumors and speculations about similar approaches to analysis of leukemia are addressed.
KW - leukemia
KW - molecular cytogenetics
KW - solid tumors
UR - http://www.scopus.com/inward/record.url?scp=0027751738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027751738&partnerID=8YFLogxK
M3 - Article
C2 - 8018948
AN - SCOPUS:0027751738
VL - 19
SP - 677
EP - 683
JO - Blood Cells, Molecules, and Diseases
JF - Blood Cells, Molecules, and Diseases
SN - 1079-9796
IS - 3
ER -