Molecular cytogenetic analysis of consistent abnormalities at 8q12-q22 in breast cancer

Marlena Schoenberg Fejzo, Tony Godfrey, Chira Chen, Fred Waldman, Joe Gray

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Studies using comparative genomic hybridization (CGH) indicate that portions of chromosome arm 8q from 8q12 to 8qter are present at an increased relative copy number in a broad range of solid tumors. In this study we define an ~ 1 Mb wide region that appears to be frequently abnormal in copy number or structure in breast cancer cell lines and primary tumors. This was accomplished by fluorescence in situ hybridization (FISH) with yeast artificial chromosomes (YACs) mapped to 8q12-q22. Eleven breast cancer cell lines and ten primary tumors were analyzed. A minimal region of rearrangement was localized to the CEPH-YAC 928F9 in three breast cancer cell lines with unbalanced translocation breakpoints mapping in this region. Unbalanced translocations also were detected in two primary tumors mapping between CEPH- YAC clones 890C4 and 936B3, flanking 928F9. An increased copy number in the minimal region was detected in nine cell lines and in multiple primary tumors. This suggests the possibility that a single gene mapping to 928F9 is involved in breast cancer development or progression and may be deregulated by copy number increases in some tumors and by translocation in others. Four expressed sequence tags were mapped to YAC 928F9 and analyzed for rearrangements by Southern analysis and for abnormal expression by Northern analysis.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalGenes Chromosomes and Cancer
Volume22
Issue number2
DOIs
StatePublished - Jun 1998
Externally publishedYes

Fingerprint

Cytogenetic Analysis
Yeast Artificial Chromosomes
Breast Neoplasms
Neoplasms
Cell Line
Comparative Genomic Hybridization
Chromosome Mapping
Expressed Sequence Tags
Tumor Cell Line
Fluorescence In Situ Hybridization
Clone Cells
Chromosomes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Molecular cytogenetic analysis of consistent abnormalities at 8q12-q22 in breast cancer. / Fejzo, Marlena Schoenberg; Godfrey, Tony; Chen, Chira; Waldman, Fred; Gray, Joe.

In: Genes Chromosomes and Cancer, Vol. 22, No. 2, 06.1998, p. 105-113.

Research output: Contribution to journalArticle

Fejzo, Marlena Schoenberg ; Godfrey, Tony ; Chen, Chira ; Waldman, Fred ; Gray, Joe. / Molecular cytogenetic analysis of consistent abnormalities at 8q12-q22 in breast cancer. In: Genes Chromosomes and Cancer. 1998 ; Vol. 22, No. 2. pp. 105-113.
@article{a890c5176a4c49a3909111bc5fec8c8a,
title = "Molecular cytogenetic analysis of consistent abnormalities at 8q12-q22 in breast cancer",
abstract = "Studies using comparative genomic hybridization (CGH) indicate that portions of chromosome arm 8q from 8q12 to 8qter are present at an increased relative copy number in a broad range of solid tumors. In this study we define an ~ 1 Mb wide region that appears to be frequently abnormal in copy number or structure in breast cancer cell lines and primary tumors. This was accomplished by fluorescence in situ hybridization (FISH) with yeast artificial chromosomes (YACs) mapped to 8q12-q22. Eleven breast cancer cell lines and ten primary tumors were analyzed. A minimal region of rearrangement was localized to the CEPH-YAC 928F9 in three breast cancer cell lines with unbalanced translocation breakpoints mapping in this region. Unbalanced translocations also were detected in two primary tumors mapping between CEPH- YAC clones 890C4 and 936B3, flanking 928F9. An increased copy number in the minimal region was detected in nine cell lines and in multiple primary tumors. This suggests the possibility that a single gene mapping to 928F9 is involved in breast cancer development or progression and may be deregulated by copy number increases in some tumors and by translocation in others. Four expressed sequence tags were mapped to YAC 928F9 and analyzed for rearrangements by Southern analysis and for abnormal expression by Northern analysis.",
author = "Fejzo, {Marlena Schoenberg} and Tony Godfrey and Chira Chen and Fred Waldman and Joe Gray",
year = "1998",
month = "6",
doi = "10.1002/(SICI)1098-2264(199806)22:2<105::AID-GCC4>3.0.CO;2-0",
language = "English (US)",
volume = "22",
pages = "105--113",
journal = "Genes Chromosomes and Cancer",
issn = "1045-2257",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Molecular cytogenetic analysis of consistent abnormalities at 8q12-q22 in breast cancer

AU - Fejzo, Marlena Schoenberg

AU - Godfrey, Tony

AU - Chen, Chira

AU - Waldman, Fred

AU - Gray, Joe

PY - 1998/6

Y1 - 1998/6

N2 - Studies using comparative genomic hybridization (CGH) indicate that portions of chromosome arm 8q from 8q12 to 8qter are present at an increased relative copy number in a broad range of solid tumors. In this study we define an ~ 1 Mb wide region that appears to be frequently abnormal in copy number or structure in breast cancer cell lines and primary tumors. This was accomplished by fluorescence in situ hybridization (FISH) with yeast artificial chromosomes (YACs) mapped to 8q12-q22. Eleven breast cancer cell lines and ten primary tumors were analyzed. A minimal region of rearrangement was localized to the CEPH-YAC 928F9 in three breast cancer cell lines with unbalanced translocation breakpoints mapping in this region. Unbalanced translocations also were detected in two primary tumors mapping between CEPH- YAC clones 890C4 and 936B3, flanking 928F9. An increased copy number in the minimal region was detected in nine cell lines and in multiple primary tumors. This suggests the possibility that a single gene mapping to 928F9 is involved in breast cancer development or progression and may be deregulated by copy number increases in some tumors and by translocation in others. Four expressed sequence tags were mapped to YAC 928F9 and analyzed for rearrangements by Southern analysis and for abnormal expression by Northern analysis.

AB - Studies using comparative genomic hybridization (CGH) indicate that portions of chromosome arm 8q from 8q12 to 8qter are present at an increased relative copy number in a broad range of solid tumors. In this study we define an ~ 1 Mb wide region that appears to be frequently abnormal in copy number or structure in breast cancer cell lines and primary tumors. This was accomplished by fluorescence in situ hybridization (FISH) with yeast artificial chromosomes (YACs) mapped to 8q12-q22. Eleven breast cancer cell lines and ten primary tumors were analyzed. A minimal region of rearrangement was localized to the CEPH-YAC 928F9 in three breast cancer cell lines with unbalanced translocation breakpoints mapping in this region. Unbalanced translocations also were detected in two primary tumors mapping between CEPH- YAC clones 890C4 and 936B3, flanking 928F9. An increased copy number in the minimal region was detected in nine cell lines and in multiple primary tumors. This suggests the possibility that a single gene mapping to 928F9 is involved in breast cancer development or progression and may be deregulated by copy number increases in some tumors and by translocation in others. Four expressed sequence tags were mapped to YAC 928F9 and analyzed for rearrangements by Southern analysis and for abnormal expression by Northern analysis.

UR - http://www.scopus.com/inward/record.url?scp=0031968156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031968156&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1098-2264(199806)22:2<105::AID-GCC4>3.0.CO;2-0

DO - 10.1002/(SICI)1098-2264(199806)22:2<105::AID-GCC4>3.0.CO;2-0

M3 - Article

C2 - 9598797

AN - SCOPUS:0031968156

VL - 22

SP - 105

EP - 113

JO - Genes Chromosomes and Cancer

JF - Genes Chromosomes and Cancer

SN - 1045-2257

IS - 2

ER -