Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues.

Charles Roberts, S. R. Lasky, W. L. Lowe, W. T. Seaman, D. LeRoith

Research output: Contribution to journalArticle

242 Citations (Scopus)

Abstract

Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 3'-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalMolecular endocrinology (Baltimore, Md.)
Volume1
Issue number3
StatePublished - Mar 1987
Externally publishedYes

Fingerprint

Molecular Cloning
Insulin-Like Growth Factor I
Growth Hormone
RNA
Complementary DNA
DNA
Messenger RNA
3' Untranslated Regions
Base Pairing
Northern Blotting
Amino Acid Sequence
Clone Cells
Liver
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

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