Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids

differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues.

Charles Roberts, S. R. Lasky, W. L. Lowe, W. T. Seaman, D. LeRoith

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 3'-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalMolecular endocrinology (Baltimore, Md.)
Volume1
Issue number3
StatePublished - Mar 1987
Externally publishedYes

Fingerprint

Molecular Cloning
Insulin-Like Growth Factor I
Growth Hormone
RNA
Complementary DNA
DNA
Messenger RNA
3' Untranslated Regions
Base Pairing
Northern Blotting
Amino Acid Sequence
Clone Cells
Liver
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{cb6a3a9b80ec488096277871430b9743,
title = "Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues.",
abstract = "Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 3'-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.",
author = "Charles Roberts and Lasky, {S. R.} and Lowe, {W. L.} and Seaman, {W. T.} and D. LeRoith",
year = "1987",
month = "3",
language = "English (US)",
volume = "1",
pages = "243--248",
journal = "Molecular Endocrinology",
issn = "0888-8809",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids

T2 - differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues.

AU - Roberts, Charles

AU - Lasky, S. R.

AU - Lowe, W. L.

AU - Seaman, W. T.

AU - LeRoith, D.

PY - 1987/3

Y1 - 1987/3

N2 - Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 3'-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.

AB - Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 3'-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.

UR - http://www.scopus.com/inward/record.url?scp=0023298435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023298435&partnerID=8YFLogxK

M3 - Article

VL - 1

SP - 243

EP - 248

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 3

ER -