Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: Differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues

Charles T. Roberts, Stephen R. Lasky, William L. Lowe, William T. Seaman, Derek Leroith

    Research output: Contribution to journalArticle

    244 Scopus citations

    Abstract

    Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 373x2032;-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.

    Original languageEnglish (US)
    Pages (from-to)243-248
    Number of pages6
    JournalMolecular Endocrinology
    Volume1
    Issue number3
    DOIs
    StatePublished - Mar 1987

    ASJC Scopus subject areas

    • Molecular Biology
    • Endocrinology

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