Molecular cloning of biologically active Rauscher spleen focus-forming virus and the sequences of its env gene and long terminal repeat

R. K. Bestwick, B. A. Boswell, David Kabat

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Rauscher and Friend spleen focus-forming viruses (R- and F-SFFVs) cause similar progressive erythroleukemias dependent upon a virus-encoded membrane glycoprotein. Moreover, these SFFV glycoproteins are immunologically related to each other and to the recombinant-type glycoproteins encoded by the env genes of dual tropic murine leukemia viruses. To better understand these diseases and the viral origins, we isolated a pathogenically active molecular clone of R-SFFV proviral DNA, sequenced its 3'-terminal 2,163-base-pair (bp) region, and compared these sequences with previously determined sequences of F-SFFV. The 516-bp R-SFFV long terminal repeat is highly homologous to those of F-SFFV and Friend murine leukemia virus, although only the latter contains a 65-bp direct repeat in its U3 region. The env gene of R-SFFV encodes a glycoprotein with 408 amino acids that is identical in its basic domain organization to the glycoprotein of F-SFFV. Thus, the junctions between the dual tropic-related and ecotropic sequences occur at the same nucleotide, and both SFFV env genes contain identical 585-bp deletions in their ecotropic domains and single-bp insertions which cause premature terminations at the same amino acid in their ecotropic p15E domains. Consistent with their independent origins, however, the env sequences of R- and F-SFFV are distinctive in both their 5' dual tropic-related and 3' ecotropic-related domains. Furthermore, there are several consistent amino acid differences between the polycythemic F-SFFV sequences and the anemia-inducing R-SFFV sequence. The striking similarities of the independently formed F- and R-SFFV env genes imply that all of the glycoprotein domains arranged in a precise organization may be required for its leukemogenic activity.

Original languageEnglish (US)
Pages (from-to)695-705
Number of pages11
JournalJournal of Virology
Volume51
Issue number3
StatePublished - 1984

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Spleen Focus-Forming Viruses
env Genes
terminal repeat sequences
Terminal Repeat Sequences
Molecular Cloning
glycoproteins
molecular cloning
spleen
viruses
Murine leukemia virus
tropics
genes
amino acids
Base Pairing
membrane glycoproteins
Glycoproteins
anemia
nucleotides
clones
Amino Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular cloning of biologically active Rauscher spleen focus-forming virus and the sequences of its env gene and long terminal repeat. / Bestwick, R. K.; Boswell, B. A.; Kabat, David.

In: Journal of Virology, Vol. 51, No. 3, 1984, p. 695-705.

Research output: Contribution to journalArticle

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