TY - JOUR
T1 - Molecular cloning and tissue distribution of a putative member of the rat opioid receptor gene family that is not a μ, δ or κ opioid receptor type
AU - Bunzow, James R.
AU - Saez, Carmen
AU - Mortrud, Marty
AU - Bouvier, Claudia
AU - Williams, John T.
AU - Low, Malcolm
AU - Grandy, David K.
N1 - Funding Information:
Acknowledgements: We would like to thankA . Mansour,S .J. Watson, Jr. and T.F. Murray for performingb indinge xperimentosn LC132.W e also would like to recognizeth ee xcellentte chnicaal ssistancper ovided by Shoko Hagen,A nja Unteutscha nd Zhu Wei Zhu. The i&&rations werep reparedw ith thea ssistancoef June Shiigia ndJ udah Askew.T his work was funded,i n part, by grantsf rom NIMH (MH48991)N, IDA (DA08562)a nd the AmericanP arkinson’sD iseaseA ssociation.
PY - 1994/6/27
Y1 - 1994/6/27
N2 - A novel G protein-coupled receptor was cloned by PCR and homology screening. Its deduced amino acid sequence is 47% identical overall to the μ, δ and κ opioid receptors and 64% identical in the putative transmembrane domains. When transiently expressed in COS-7 cells this receptor did not bind any of the typical μ, δ or κ opioid receptor ligands with high affinity. In situ hybridization analysis revealed that LC132 mRNA is highly expressed in several rat brain areas, including the cerebral cortex, thalamus, subfornical organ, habenula, hypothalamus, central gray, dorsal raphe, locus coeruleus and the dorsal horn of the spinal cord. Based on this distribution and its high homology with the μ, δ and κ opioid receptors, it is proposed that LC132 is a new member of the opioid receptor family that is involved in analgesia and the perception of pain.
AB - A novel G protein-coupled receptor was cloned by PCR and homology screening. Its deduced amino acid sequence is 47% identical overall to the μ, δ and κ opioid receptors and 64% identical in the putative transmembrane domains. When transiently expressed in COS-7 cells this receptor did not bind any of the typical μ, δ or κ opioid receptor ligands with high affinity. In situ hybridization analysis revealed that LC132 mRNA is highly expressed in several rat brain areas, including the cerebral cortex, thalamus, subfornical organ, habenula, hypothalamus, central gray, dorsal raphe, locus coeruleus and the dorsal horn of the spinal cord. Based on this distribution and its high homology with the μ, δ and κ opioid receptors, it is proposed that LC132 is a new member of the opioid receptor family that is involved in analgesia and the perception of pain.
KW - Analgesia
KW - In situ hybridization
KW - Opioid receptor
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U2 - 10.1016/0014-5793(94)00561-3
DO - 10.1016/0014-5793(94)00561-3
M3 - Article
C2 - 8034019
AN - SCOPUS:0028284167
SN - 0014-5793
VL - 347
SP - 284
EP - 288
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -