Molecular cloning and tissue distribution of a putative member of the rat opioid receptor gene family that is not a μ, δ or κ opioid receptor type

James R. Bunzow, Carmen Saez, Marty Mortrud, Claudia Bouvier, John T. Williams, Malcolm Low, David K. Grandy

Research output: Contribution to journalArticle

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Abstract

A novel G protein-coupled receptor was cloned by PCR and homology screening. Its deduced amino acid sequence is 47% identical overall to the μ, δ and κ opioid receptors and 64% identical in the putative transmembrane domains. When transiently expressed in COS-7 cells this receptor did not bind any of the typical μ, δ or κ opioid receptor ligands with high affinity. In situ hybridization analysis revealed that LC132 mRNA is highly expressed in several rat brain areas, including the cerebral cortex, thalamus, subfornical organ, habenula, hypothalamus, central gray, dorsal raphe, locus coeruleus and the dorsal horn of the spinal cord. Based on this distribution and its high homology with the μ, δ and κ opioid receptors, it is proposed that LC132 is a new member of the opioid receptor family that is involved in analgesia and the perception of pain.

Original languageEnglish (US)
Pages (from-to)284-288
Number of pages5
JournalFEBS Letters
Volume347
Issue number2-3
DOIs
StatePublished - Jun 27 1994

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Keywords

  • Analgesia
  • In situ hybridization
  • Opioid receptor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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